Growth regulator requirement for in vitro embryogenic cultures of snowdrop (Galanthus nivalis L.) suitable for germplasm preservation
| Autor: | Gyula Surányi, Ágnes Mosolygó, Zita Demeter, Sándor Gonda, Emese Ficsor, Éva Szőke, A. Balázs, Csaba Máthé, László Papp, Anna Resetár |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Germplasm
Somatic embryogenesis Genotype Plant Development Biológiai tudományok Biology Plant Roots General Biochemistry Genetics and Molecular Biology Naphthaleneacetic Acids Tissue Culture Techniques Tissue culture chemistry.chemical_compound Plant Growth Regulators Természettudományok Auxin Gene Expression Regulation Plant Botany Benzyl Compounds Regeneration General Environmental Science chemistry.chemical_classification Galanthus Indoleacetic Acids fungi food and beverages Kinetin biology.organism_classification Phenotype Neurology chemistry Purines Callus Cytokinin Seeds Plant Shoots Galanthus nivalis Explant culture |
| Popis: | In this study, we report on the production of bulb scale-derived tissue cultures capable of efficient shoot and plant regeneration in three genotypes of snowdrop (Galanthus nivalis L., Amaryllidaceae), a protected ornamental plant. For culture line A, high auxin and low cytokinin concentration is required for callus production and plant regeneration. The type of auxin is of key importance: α-naphthaleneacetic acid (NAA) in combination with indole-3-acetic acid (IAA) at concentrations of 2 mg L-1 or 2-10 mg L-1 NAA with 1 mg L-1 N6-benzyladenine (BA), a cytokinin on full-strength media are required for regeneration. Cultures showing regeneration were embryogenic. When lines B and C were induced and maintained with 2 mg L-1 NAA and 1 mg L-1 BA, they produced mature bulblets with shoots, without roots. Line A produced immature bulblets with shoots under the above culture condition. Amplified Fragment Length Polymorphism (AFLP) analysis showed that (i) genetic differences between line A and its bulb explants were not significant, therefore these tissue cultures are suitable for germplasm preservation, and (ii) different morphogenetic responses of lines A, B and C originated from genetic differences. Culture line A is suitable for field-growing, cultivation and germplasm preservation of G. nivalis and for the production of Amaryllidaceae alkaloids. |
| Databáze: | OpenAIRE |
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