Endogenous substrates for cyclic AMP-dependent and calcium-dependent protein phosphorylation in rabbit peritoneal neutrophils
| Autor: | Barbara-Jean Bormann, William M. Mackin, Chi-Kuang Huang, John M. Hill, Elmer L. Becker |
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| Rok vydání: | 1983 |
| Předmět: |
MAP kinase kinase kinase
biology Neutrophils Cyclin-dependent kinase 2 Biophysics Blood Proteins Mitogen-activated protein kinase kinase Biochemistry Molecular biology MAP2K7 Kinetics biology.protein Cyclic AMP Animals Calcium c-Raf Rabbits Phosphorylation Protein kinase A Molecular Biology cGMP-dependent protein kinase Protein Kinases Protein kinase C Protein Kinase C |
| Zdroj: | Biochimica et biophysica acta. 760(1) |
| ISSN: | 0006-3002 |
| Popis: | As in other cells, cAMP-dependent (protein kinase A) and calcium-dependent protein kinases are present in the rabbit peritoneal neutrophil. The major substrates for protein kinase A in the cytosol of rabbit peritoneal neutrophil is a 43 kDa protein which appears to be actin (p I 5.7). The other substrates for protein kinase A in the cytosol are very acidic proteins with molecular weights of 135 000 (p I 4.6) and 130 000 (p I 4.8). Two classes of calcium-dependent protein kinases are present in the rabbit peritoneal neutrophil: one is calcium, calmodulin-dependent, the other is calcium, phosphatidylserine-dependent. Phosphatidylserine appears to be much more effective than calmodulin in stimulting calcium-dependent protein kinase activity. The phospolipid-sensitive, calcium-dependent protein kinase (protein kinase C), present only in the cytosol fraction, exhibits much higher activity than the cAMP-dependent protein kinase from the same source. At least four substrates (M r 130 000 (p I 4.6) 43 000 (p I 4.8), 41 000 (p I 6.3) and 34 000) of the protein kinase C in the cytosol were identified. Trifluoperazine, a compound which inhibits the degranulation, aggregation and stimulated oxygen consumption of rabbit peritoneal neutrophils. (Alobaidi, T., Naccache, P.H. and Sha'afi, R.I. (1981) Biochim. Biophys. Acta 675, 316–321), also inhibits the activity of protein kinase C. The possible role of cAMP-dependent and calcium-dependent phosphorylation system in neutrophil function is discussed. |
| Databáze: | OpenAIRE |
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