Nonmuscle myosin II-B is required for normal development of the mouse heart
| Autor: | Antonella N. Tullio, Zu-Xi Yu, Domenico Accili, Heiner Westphal, Alexander Grinberg, Robert S. Adelstein, Kazuyo Takeda, Yvette A. Preston, Victor J. Ferrans |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
medicine.medical_specialty
Mice Transgenic Biology Mice Double outlet right ventricle Internal medicine Myosin MYH10 medicine Animals Humans Myocyte Tetralogy of Fallot Nonmuscle Myosin Type IIB Multidisciplinary Myosin Heavy Chains Myocardium Cardiac myocyte Gene Expression Regulation Developmental Heart Anatomy Biological Sciences medicine.disease Endocrinology medicine.anatomical_structure Animals Newborn Ventricle Heart failure Gene Deletion |
| Zdroj: | Proceedings of the National Academy of Sciences. 94:12407-12412 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.94.23.12407 |
| Popis: | We used targeted gene disruption in mice to ablate nonmuscle myosin heavy chain B (NMHC-B), one of the two isoforms of nonmuscle myosin II present in all vertebrate cells. Approximately 65% of the NMHC-B −/− embryos died prior to birth, and those that were born suffered from congestive heart failure and died during the first day. No abnormalities were detected in NMHC-B +/− mice. The absence of NMHC-B resulted in a significant increase in the transverse diameters of the cardiac myocytes from 7.8 ± 1.8 μm (right ventricle) and 7.8 ± 1.3 μm (left ventricle) in NMHC-B +/+ and B +/− mice to 14.7 ± 1.1 μm and 13.8 ± 2.3 μm, respectively, in NMHC-B −/− mice (in both cases, P < 0.001). The increase in size of the cardiac myocytes was seen as early as embryonic day 12.5 (4.5 ± 0.2 μm for NMHC-B +/+ and B +/− vs. 7.2 ± 0.6 μm for NMHC-B −/− mice ( P < 0.01)). Six of seven NMHC-B −/− newborn mice analyzed by serial sectioning also showed structural cardiac defects, including a ventricular septal defect, an aortic root that either straddled the defect or originated from the right ventricle, and muscular obstruction to right ventricular outflow. Some of the hearts of NMHC-B −/− mice showed evidence for up-regulation of NMHC-A protein. These studies suggest that nonmuscle myosin II-B is required for normal cardiac myocyte development and that its absence results in structural defects resembling, in part, two common human congenital heart diseases, tetralogy of Fallot and double outlet right ventricle. |
| Databáze: | OpenAIRE |
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