Enhanced solvent extraction of polar lipids associated with rubber particles from Hevea brasiliensis
| Autor: | Jérôme Sainte-Beuve, Christian Aymard, Eugene Ejolle Ehabe, Laurent Vaysse, Frédéric Bonfils |
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| Rok vydání: | 2007 |
| Předmět: |
Latex
Plant Science Biochemistry Analytical Chemistry Méthanol Drug Discovery Immersion Solubility Phospholipids biology Lipide Chemistry Temperature General Medicine Phosphatide Lipids Thin-layer chromatography Fluid extract Acétone Hevea brasiliensis visual_art Extraction par solvant visual_art.visual_art_medium Molecular Medicine lipids (amino acids peptides and proteins) Chloroform Acetone Adsorption Teneur en lipides Natural rubber Q60 - Traitement des produits agricoles non alimentaires Chromatography Caoutchouc Methanol Extraction (chemistry) Glycolipide biology.organism_classification Complementary and alternative medicine Solvents Particle Hevea Chloroforme Chromatography Thin Layer Glycolipids Food Science |
| Zdroj: | Phytochemical Analysis |
| ISSN: | 0958-0344 |
| Popis: | Biochemical studies of lipids bound to rubber particles have been complicated due to the solubility of polyisoprene chains in most extracting solvents and the rather delicate nature of polar lipids that arc often denatured when traditional solvent extraction techniques are employed. In this paper, we describe a traditional technique and accompanying solvents that permit optimal extraction of rubber particle bound lipids. The technique, which is validated after characterizing the lipid extracts by elemental analysis, silica column adsorption and thin layer chromatography, appeared more suitable for extracting total lipids with optimal glycolipid and phospholipid contents. This technique is proposed as an alternative to traditional extraction methods used for solid natural rubber as it offers advantages with respect to ease of application, extract quality, extraction yields and reproducibility. Copyright. © 2007 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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