A stalled-ribosome rescue factor Pth3 is required for mitochondrial translation against antibiotics in Saccharomyces cerevisiae
| Autor: | Daisuke Kurita, Soichiro Hoshino, Nobukazu Nameki, Yukihiro Soutome, Ryohei Kanemura, Masakatsu Watanabe, Hyouta Himeno, Masak Takaine |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Ribosomal Proteins
0301 basic medicine Mitochondrial DNA Saccharomyces cerevisiae Proteins QH301-705.5 Mitochondrial translation Saccharomyces cerevisiae Medicine (miscellaneous) Biology Ribosome Article General Biochemistry Genetics and Molecular Biology Mitochondrial Proteins Mitochondrial Ribosomes 03 medical and health sciences 0302 clinical medicine Drug Resistance Fungal Antibiotics Gene Expression Regulation Fungal Mitochondrial ribosome RNA Messenger Biology (General) Gene Messenger RNA Fungi RNA Fungal biology.organism_classification Stop codon Anti-Bacterial Agents Cell biology 030104 developmental biology Protein Biosynthesis Mutation General Agricultural and Biological Sciences 030217 neurology & neurosurgery Peptide Termination Factors |
| Zdroj: | Communications Biology Communications Biology, Vol 4, Iss 1, Pp 1-11 (2021) |
| ISSN: | 2399-3642 |
| DOI: | 10.1038/s42003-021-01835-6 |
| Popis: | Mitochondrial translation appears to involve two stalled-ribosome rescue factors (srRFs). One srRF is an ICT1 protein from humans that rescues a “non-stop” type of mitochondrial ribosomes (mitoribosomes) stalled on mRNA lacking a stop codon, while the other, C12orf65, reportedly has functions that overlap with those of ICT1; however, its primary role remains unclear. We herein demonstrated that the Saccharomyces cerevisiae homolog of C12orf65, Pth3 (Rso55), preferentially rescued antibiotic-dependent stalled mitoribosomes, which appear to represent a “no-go” type of ribosomes stalled on intact mRNA. On media containing a non-fermentable carbon source, which requires mitochondrial gene expression, respiratory growth was impaired significantly more by the deletion of PTH3 than that of the ICT1 homolog PTH4 in the presence of antibiotics that inhibit mitochondrial translation, such as tetracyclines and macrolides. Additionally, the in organello labeling of mitochondrial translation products and quantification of mRNA levels by quantitative RT-PCR suggested that in the presence of tetracycline, the deletion of PTH3, but not PTH4, reduced the protein expression of all eight mtDNA-encoded genes at the post-transcriptional or translational level. These results indicate that Pth3 can function as a mitochondrial srRF specific for ribosomes stalled by antibiotics and plays a role in antibiotic resistance in fungi. Hoshino et al. show that PTH3, the S. cerevisiae homolog of C12orf65, is required for the translation of mitochondrial genes in the presence of antibiotics. This study suggests that PTH3 rescues the ribosomes stalled by antibiotics, playing a role in antibiotic resistance in fungi. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink