Resistance detection of blackjack to ALS inhibitors by in vitro plant growth method
| Autor: | Lais Tessari Perboni, J.R. Garcia, Daniela Tessaro, Daiane Benneman, Marlon M. Teixeira, Dirceu Agostinetto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Physiology QH301-705.5 Greenhouse Plant Science Biology 01 natural sciences Biochemistry Tissue culture Bidens Pilosa Biology (General) resistance test Acetolactate synthase Resistance (ecology) business.industry fungi Botany food and beverages 04 agricultural and veterinary sciences Weed control In vitro Biotechnology acetolactate synthase QK1-989 040103 agronomy & agriculture biology.protein 0401 agriculture forestry and fisheries business Weed Agronomy and Crop Science 010606 plant biology & botany Explant culture weed |
| Zdroj: | Planta Daninha, Vol 38 (2020) Planta Daninha v.38 2020 Planta daninha Sociedade Brasileira da Ciência das Plantas Daninhas (SBCPD) instacron:SBCPD |
| ISSN: | 0100-8358 |
| Popis: | Background: The resistance of weeds to herbicides is a problem that has been increasingly studied because of its great importance in agriculture and rapid growth of this phenomenon worldwide. The quick detection of resistant plants is extremely important for resistance management. Weed resistance to herbicides can be detected through field, greenhouse and laboratory tests. A fast way of detecting resistance is using tissue culture method, where is possible to use resistant plant clones and obtain results faster than whole-plant method in greenhouse. Objective: The aims of the research were to detect the resistance of blackjack to herbicides inhibitors of the acetolactate synthase (ALS), addition in vitro cultivation; determine the herbicide concentration of imazethapyr to 95% control of the susceptible plant population; evaluate explants growth; and, determine the herbicide concentration added to the culture medium where is possible to distinguish a resistant from a susceptible plant. Methods: Experiments were carried out in greenhouse and tissue culture laboratory. Screening was performed to select resistant and susceptible biotypes, tests for specie establishment and dose response curves in vitro were made. Results: The herbicide concentration added to the culture medium that provided 95% susceptible biotype control and efficiently differentiated susceptible biotype from resistant one was 0.6μM of imazethapyr. Conclusions: This method helps recommendations of weed management and provides a quick decision to alternative control of this specie, thus avoiding major damage to the crops. |
| Databáze: | OpenAIRE |
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