Cytokinetic abscission is part of the midblastula transition in early zebrafish embryogenesis
| Autor: | Yarden M. Jahn, Assaf Zaritsky, Nadine Peyriéras, Natalie Elia, Shai Adar-Levor, Dikla Nachmias, Shani T. Gal-Oz, Ramon Y. Birnbaum |
|---|---|
| Přispěvatelé: | Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
cell division
animal structures Cell division [SDV]Life Sciences [q-bio] Biology ESCRT Midblastula microtubules 03 medical and health sciences 0302 clinical medicine Abscission MBT Animals Mitosis Zebrafish development Cell Shape 030304 developmental biology Cytokinesis 0303 health sciences Multidisciplinary Endosomal Sorting Complexes Required for Transport Embryo Cell Biology Biological Sciences Blastula Zebrafish Proteins biology.organism_classification Cell biology abscission embryonic structures 030217 neurology & neurosurgery |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2021, 118 (15), pp.e2021210118. ⟨10.1073/pnas.2021210118⟩ |
| ISSN: | 0027-8424 1091-6490 |
| DOI: | 10.1073/pnas.2021210118⟩ |
| Popis: | Significance In this work, we show that the last step of cytokinesis, termed abscission, is delayed in early zebrafish embryos. As a result, sibling cells remain connected to one another by a thin membrane bridge for several cycles, forming clusters of interconnected cells. Bridge severing (i.e., abscission) commences at the 10th cell cycle when embryos enter the midblastula transition switch, in which embryonic cells become individualized and exhibit the characteristics of mature cells. Cells connected by intercellular bridges shared similar cellular behaviors, such as transcription onset and cell shape. Our data suggest that cell–cell connectivity is maintained in early embryos through persistent bridge connections that allow cells to coordinate their behavior during embryonic development. Animal cytokinesis ends with the formation of a thin intercellular membrane bridge that connects the two newly formed sibling cells, which is ultimately resolved by abscission. While mitosis is completed within 15 min, the intercellular bridge can persist for hours, maintaining a physical connection between sibling cells and allowing exchange of cytosolic components. Although cell–cell communication is fundamental for development, the role of intercellular bridges during embryogenesis has not been fully elucidated. In this work, we characterized the spatiotemporal characteristics of the intercellular bridge during early zebrafish development. We found that abscission is delayed during the rapid division cycles that occur in the early embryo, giving rise to the formation of interconnected cell clusters. Abscission was accelerated when the embryo entered the midblastula transition (MBT) phase. Components of the ESCRT machinery, which drives abscission, were enriched at intercellular bridges post-MBT and, interfering with ESCRT function, extended abscission beyond MBT. Hallmark features of MBT, including transcription onset and cell shape modulations, were more similar in interconnected sibling cells compared to other neighboring cells. Collectively, our findings suggest that delayed abscission in the early embryo allows clusters of cells to coordinate their behavior during embryonic development. |
| Databáze: | OpenAIRE |
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