Stenotrophomonas maltophiliaouter membrane vesicles elicit a potent inflammatory responsein vitroandin vivo

Autor: Sang Hwa Lee, Hyo Il Kwon, Yoo Jeong Kim, Seok Hyeon Na, Hyejin Jeon, Je Chul Lee, Asiimwe Nicholas, Tae In Park, Gati Noble Selasi
Rok vydání: 2016
Předmět:
0301 basic medicine
Microbiology (medical)
Chemokine
Stenotrophomonas maltophilia
medicine.medical_treatment
030106 microbiology
Gene Expression
Cell Line
Microbiology
Mice
03 medical and health sciences
polycyclic compounds
medicine
Animals
Humans
Immunology and Allergy
Secretion
Interleukin 8
Transport Vesicles
Inflammation
A549 cell
General Immunology and Microbiology
biology
Secretory Vesicles
Monocyte
Cell Membrane
Epithelial Cells
General Medicine
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
biology.organism_classification
respiratory tract diseases
Disease Models
Animal
Infectious Diseases
medicine.anatomical_structure
Cytokine
biology.protein
Cytokines
bacteria
Female
Inflammation Mediators
Gram-Negative Bacterial Infections
Bacterial outer membrane
Zdroj: Pathogens and Disease. 74:ftw104
ISSN: 2049-632X
Popis: Stenotrophomonas maltophilia has become one of the most prevalent opportunistic pathogens in hospitalized patients. This microorganism secretes outer membrane vesicles (OMVs), but the pathogenesis of S. maltophilia as it relates to OMVs has not been characterized. This study investigated the cytotoxic activity of S. maltophilia OMVs and their ability to induce inflammatory responses both in vitro and in vivo Stenotrophomonas maltophilia ATCC 13637 and two clinical isolates were found to secrete spherical OMVs during in vitro culture. OMVs from S. maltophilia ATCC 13637 were cytotoxic to human lung epithelial A549 cells. Stenotrophomonas maltophilia OMVs stimulated the expression of proinflammatory cytokine and chemokine genes, including interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor-α and monocyte chemoattractant protein-1, in A549 cells. Early inflammatory responses such as congestion and neutrophilic infiltrations and profound expression of proinflammatory cytokine and chemokine genes were observed in the lungs of mice injected with S. maltophilia OMVs, and were similar to responses elicited by the bacteria. Our data demonstrate that S. maltophilia OMVs are important secretory nanocomplexes that elicit a potent inflammatory response that might contribute to S. maltophilia pathogenesis during infection.
Databáze: OpenAIRE
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