Sister-chromatid exchange in highly purified human CD4+ and CD8+ lymphocytes
| Autor: | Gilbert B. Côté, Maria Grigoriadou, Constantin N. Baxevanis, Michael Papamichail, George J. Reclos, Theodor Spanos, Marilena Papanastasiou, Emi Sarafidou, Catherine Sarri |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
CD4-Positive T-Lymphocytes
CD8 Antigens Mitomycin T-Lymphocytes Health Toxicology and Mutagenesis Lymphocyte Sister chromatid exchange Biology Lymphocyte Activation Flow cytometry chemistry.chemical_compound T-Lymphocyte Subsets Immunogenetics Genetics medicine Humans Phytohemagglutinins Molecular Biology Incubation Cells Cultured medicine.diagnostic_test Mitomycin C T lymphocyte Molecular biology medicine.anatomical_structure Bromodeoxyuridine chemistry CD4 Antigens Immunology Sister Chromatid Exchange Cell Division CD8 |
| Zdroj: | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 270:125-133 |
| ISSN: | 0027-5107 |
| DOI: | 10.1016/0027-5107(92)90123-j |
| Popis: | Sister-chromatid exchange (SCE) frequencies were determined in human peripheral blood CD4+ and CD8+ T lymphocyte subpopulations which were rapidly and highly purified from pooled T lymphocytes by immunological methods. The purified lymphocytes were stimulated with phytohemagglutinin (PHA) for 4 days. CD4+ lymphocytes showed significantly higher SCE frequencies than autologous CD8+ lymphocytes when measured simultaneously after identical bromodeoxyuridine (BrdU) incubation times. Differences in SCE frequencies between CD4+ and CD8+ lymphocytes were also detected when mitomycin C (MMC) was added to the cultures. Higher SCE frequencies in CD4+ lymphocytes were associated with lower proliferating rate indices (PRI) as compared to autologous CD8+ lymphocytes. Abnormalities in CD4+ T lymphocyte function and number in peripheral blood have been observed in several diseases characterized by immunological disorders. Thus, our data may suggest a link between some immunological disturbances and abnormal SCE frequencies in T lymphocyte subsets. |
| Databáze: | OpenAIRE |
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