Fine analysis of the Pneumocystis carinii f. sp. carinii genome by two-dimensional pulsed-field gel electrophoresis
| Autor: | Emmanuel Cornillot, Brian C. Keller, Christian P. Vivarès, Melanie T. Cushion, Guy Méténier |
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| Rok vydání: | 2002 |
| Předmět: |
Genetics
Pneumocystis Restriction Mapping Fungal genetics Karyotype General Medicine Biology Molecular biology Genome Electrophoresis Gel Pulsed-Field Restriction map Bacterial Proteins Pneumocystis carinii Electrophoresis Gel Two-Dimensional Chromosomes Fungal Genome Fungal Restriction fragment length polymorphism Ploidy DNA Fungal Deoxyribonucleases Type II Site-Specific Gene |
| Zdroj: | Gene. 293:87-95 |
| ISSN: | 0378-1119 |
| Popis: | Pneumocystis carinii is a general designation for a group of unusual unicellular fungal parasites responsible of pneumopathy in animal hosts. Divided into several subgroups termed the 'special forms', P. carinii is prone to an extensive karyotype variation. In previous studies, the nuclear genome of these organisms has been considered to be haploid and a set of 16 chromosomes has been assigned to P. carinii f. sp. carinii, a special form known to infect rats. We report the analysis of the genome of an isolate representative of the karyotype 1 of this special form, using two-dimensional pulsed-field gel electrophoresis procedures. The 'karyotype and restriction display' (KARD) fingerprints indicated the presence of 17 different chromosomes. The haploid genome size was estimated to be 8.4 Mbp. Some homologous chromosomes were distinguished on the basis of a single restriction fragment length polymorphism, which raises the possibility of a diploid nucleus. A restriction map of the chromosome 15, characterized by two homologues with a size difference of 7 kb, was constructed. Hybridization data indicated that insertion/deletion events may have occurred within subtelomeric regions which carry genes encoding the major surface glycoprotein (MSG) of Pneumocystis. |
| Databáze: | OpenAIRE |
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