Inhibition of p-Aminophenol Metabolism: a Possible Mechanism of Enhancement of Aniline Hydroxylation
| Autor: | Karl M. Oberholser, Frank E. Greene, Magdi R.I. Soliman, Terry R. Brown, James T. Stevens |
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| Rok vydání: | 1980 |
| Předmět: |
Male
Cytochrome In Vitro Techniques Aminophenols Hydroxylation chemistry.chemical_compound Aniline Malaoxon Cytochrome b5 medicine Animals Pharmacology Aniline Compounds Paraoxon biology General Medicine Metabolism Diet Rats Kinetics chemistry Biochemistry Microsomes Liver Microsome biology.protein Cytochromes Oxidation-Reduction medicine.drug |
| Zdroj: | Pharmacology. 21:153-160 |
| ISSN: | 1423-0313 0031-7012 |
| DOI: | 10.1159/000137427 |
| Popis: | The p-hydroxylation of aniline has been traditionally determined by measurement of p-aminophenol (PAP) formation. Comparison of isotopic and colorimetric procedures indicate that the actual amount of aniline metabolized exceeds the among of PAP recovered. Data suggest that enhancement of p-hydroxylation of aniline by acetone, malaoxon and paraoxon may result from inhibition of further metabolism of PAP by the microsomal cytochrome b5-dependent desaturase system. Involvement of the desaturase system is supported by observations that: (a) metabolism of PAP was reduced by starvation and stimulated when starvation was followed by feeding a high carbohydrate diet; (b) enrichment of hepatic microsomes with detergent purified cytochrome b5 decreased the amount of aniline apparently metabolized, as measured by the amount of PAP recovered, and (c) a high correlation occurred between effects of acetone, malaoxon and paraoxon on reoxidation of cytochrome b5 and capacity of these three compounds to enchance aniline metabolism. |
| Databáze: | OpenAIRE |
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