Establishment of a trimodality analytical platform for tracing, imaging and quantification of gold nanoparticles in animals by radiotracer techniques
| Autor: | Chung Shi Yang, Sih Yu Chen, Wei Neng Liao, Mei-Ya Wang, Min-Hua Chen, Mo Hsiung Yang, Su Chin Huang, Yo Wen Chen, Fong Sian Lin, Jinn Jer Peir, Jen Kun Chen, Chien-Hung Chen, Fong In Chou, Dueng-Yuan Hueng, Ming Hua Hsu, Wan Yu Lin, Ching Ya Chen, Sanching L. Liang, Yeukuang Hwu |
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| Rok vydání: | 2014 |
| Předmět: |
Diagnostic Imaging
Male Bioanalysis Mice Inbred ICR Biocompatibility Metal Nanoparticles Nanotechnology Polyethylene glycol Analytical Chemistry Polyethylene Glycols chemistry.chemical_compound Mice chemistry In vivo Colloidal gold Drug delivery Surface modification Animals Tissue Distribution Gold Particle Size Radioactive Tracers Radionuclide Imaging Ex vivo Half-Life |
| Zdroj: | Analytical chemistry. 87(1) |
| ISSN: | 1520-6882 |
| Popis: | This study aims to establish a (198)Au-radiotracer technique for in vivo tracing, rapid quantification, and ex vivo visualization of PEGylated gold nanoparticles (GNPs) in animals, organs and tissue dissections. The advantages of GNPs lie in its superior optical property, biocompatibility and versatile conjugation chemistry, which are promising to develop diagnostic probes and drug delivery systems. (198)Au is used as a radiotracer because it simultaneously emits beta and gamma radiations with proper energy and half-life; therefore, (198)Au can be used for bioanalytical purposes. The (198)Au-tagged radioactive gold nanoparticles ((198)Au-GNPs) were prepared simply by irradiating the GNPs in a nuclear reactor through the (197)Au(n,γ)(198)Au reaction and subsequently the (198)Au-GNPs were subjected to surface modification with polyethylene glycol to form PEGylated (198)Au-GNPs. The (198)Au-GNPs retained physicochemical properties that were the same as those of GNP before neutron irradiation. Pharmacokinetic and biodisposition studies were performed by intravenously injecting three types of (198)Au-GNPs with or without PEGylation into mice; the γ radiation in blood specimens and dissected organs was then measured. The (198)Au-radiotracer technique enables rapid quantification freed from tedious sample preparation and shows more than 95% recovery of injected GNPs. Clinical gamma scintigraphy was proved feasible to explore spatial- and temporal-resolved biodisposition of (198)Au-GNPs in living animals. Moreover, autoradiography, which recorded beta particles from (198)Au, enabled visualizing the heterogeneous biodisposition of (198)Au-GNPs in different microenvironments and tissues. In this study, the (198)Au-radiotracer technique facilitated creating a trimodality analytical platform for tracing, quantifying and imaging GNPs in animals. |
| Databáze: | OpenAIRE |
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