Enterotoxic Effect of the Vacuolating Toxin Produced byHelicobacter pyloriin Caco‐2 Cells
| Autor: | Alfredo Guarino, Massimo Bisceglia, John L. Telford, Rino Rappuoli, Roberto Berni Canani, Paola Massari, Giuseppe Mallardo, M. C. Boccia, Eugenia Bruzzese |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Bacterial Toxins
Enterotoxin Biology medicine.disease_cause Microbiology chemistry.chemical_compound Bacterial Proteins BAPTA Electric Impedance medicine Humans Immunology and Allergy Cytotoxicity Egtazic Acid Dose-Response Relationship Drug Helicobacter pylori Cytotoxins Toxin Biological Transport bacterial infections and mycoses biology.organism_classification digestive system diseases Epithelium Intestines Infectious Diseases medicine.anatomical_structure chemistry Caco-2 Cell culture Vacuoles bacteria Indicators and Reagents Caco-2 Cells |
| Zdroj: | The Journal of Infectious Diseases. 178:1373-1378 |
| ISSN: | 1537-6613 0022-1899 |
| Popis: | Preliminary clinical evidence suggests that Helicobacter pylori may be associated with diarrhea through its vacuolating toxin (VacA). To establish whether VacA induces intestinal secretion, epithelial damage, or both, purified pH-activated VacA was added to Caco-2 cell monolayers mounted in Ussing chambers, and electrical parameters were monitored. Mucosal addition of VacA induced an increase in short circuit current, consistent with enterotoxic effect. The effect was time- and dose-dependent and saturable. It was not found if the toxin was not pH-activated, added to the serosal side, or preheated. In cells preloaded with the Ca2+ buffering compound BAPTA/AM or with the Cl- channel inhibitor 5-nitro-2-3-(3-phenylpropylamino)benzoic acid, short circuit current did not change, indicating that VacA induces activation of Ca2+-dependent Cl- channels. VacA did not show cytopathic effects, as judged by tissue resistance. These results support the hypothesis that H. pylori may be associated with diarrhea through production of VacA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|