A single complementary-sense transcript of a geminiviral DNA beta satellite is determinant of pathogenicity
| Autor: | S. A. Akbar Behjatnia, Shahida Hasnain, M. Ali Rezaian, Shahid Mansoor, Yusuf Zafar, Muhammad Saeed |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Physiology
Satellite DNA TATA box Molecular Sequence Data DNA Satellite medicine.disease_cause chemistry.chemical_compound Open Reading Frames Start codon Tobacco medicine Amino Acid Sequence RNA Messenger Genetics Mutation Gossypium biology Base Sequence General Medicine biology.organism_classification Plants Genetically Modified Molecular biology Stop codon Open reading frame Geminiviridae chemistry DNA Viral RNA Viral Cauliflower mosaic virus Agronomy and Crop Science DNA |
| Zdroj: | Molecular plant-microbe interactions : MPMI. 18(1) |
| ISSN: | 0894-0282 |
| Popis: | Small circular single-stranded DNA satellites, termed DNAbeta, have recently been found associated with some geminivirus infections. The DNA beta associated with Cotton leaf curl virus is responsible for symptom expression of a devastating disease in Pakistan. Mutagenesis of DNA beta revealed that the complementary-sense open reading frame (ORF) betaC1 is required for inducing disease symptoms in Nicotiana tabacum. An ORF present on the virion-sense strand betaV1 appeared to have no role in pathogenesis. Tobacco plants transformed with a betaC1 ORF under the control of the Cauliflower mosaic virus 35S promoter or with a dimeric DNA beta exhibited severe disease-like phenotypes, while plants transformed with a mutated version of betaC1 appeared normal. Northern blot analysis of RNA from the transgenic plants, using strand-specific probes, identified a single complementary-sense transcript. The transcript carries the full betaC1 ORF encoding a 118-amino acid product. It maps to the DNA beta at nucleotide position 186 to 563 and contains a polyadenylation signal 18 nt upstream of the stop codon. A TATA box is located 43 nt upstream of the start codon. Our results indicate that betaC1 protein is responsible for DNA beta-induced disease symptoms. |
| Databáze: | OpenAIRE |
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