Drosophila Med6 Is Required for Elevated Expression of a Large but Distinct Set of Developmentally Regulated Genes
| Autor: | Changwon Kang, Young Joon Kim, Byung Soo Gim, Jin Mo Park, Jeong Ho Yoon |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Transcriptional Activation
DNA Complementary Saccharomyces cerevisiae Proteins Time Factors Transcription Genetic Cell Survival Recombinant Fusion Proteins Green Fluorescent Proteins Biology Polymerase Chain Reaction MED1 Fungal Proteins Mediator Coactivator Transcriptional regulation Animals Drosophila Proteins Tissue Distribution Cloning Molecular Promoter Regions Genetic Molecular Biology Alleles Polymorphism Single-Stranded Conformational Locus control region Oligonucleotide Array Sequence Analysis Cell Nucleus Transcriptional Regulation Genetics Mediator Complex General transcription factor Reverse Transcriptase Polymerase Chain Reaction Homozygote Promoter Cell Biology Blotting Northern Luminescent Proteins Phenotype Lac Operon DNA Nucleotidyltransferases Mutation Trans-Activators Nuclear receptor coactivator 2 Drosophila Cell Division |
| Zdroj: | Molecular and Cellular Biology. 21:5242-5255 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.21.15.5242-5255.2001 |
| Popis: | Mediator is the evolutionarily conserved coactivator required for the integration and recruitment of diverse regulatory signals to basal transcription machinery. To elucidate the functions of metazoan Mediator, we isolated Drosophila melanogaster Med6 mutants. dMed6 is essential for viability and/or proliferation of most cells. dMed6 mutants failed to pupate and died in the third larval instar with severe proliferation defects in imaginal discs and other larval mitotic cells. cDNA microarray, quantitative reverse transcription-PCR, and in situ expression analyses of developmentally regulated genes in dMed6 mutants showed that transcriptional activation of many, but not all, genes was affected. Among the genes found to be affected were some that play a role in cell proliferation and metabolism. Therefore, dMed6 is required in most cells for transcriptional regulation of many genes important for diverse aspects of Drosophila development. The development of multicellular organisms, even at the level of a single cell, demands a complex array of transcriptional regulation mechanisms for proper proliferation and development. To meet the demand, eukaryotic cells utilize transcriptional machinery comprising dozens of proteins that recognize and initiate RNA synthesis from promoters and that regulate the efficiency of transcription using thousands of specialized transcription factors. In addition, a number of coactivator complexes working at diverse stages of transcription add to the depth of regulatory complexity to achieve the orchestrated developmental control of gene expression in higher eukaryotes. Although these coactivator proteins appear to be required for transcriptional regulation in general, different groups of genes show different coactivator requirements. In addition, these coactivator functions are carried out by a number of complexes. Therefore, each coactivator complex appears to have unique and specific roles in transcriptional regulation of diverse developmental processes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|