Characteristics of Smooth Muscle Cell Lipoprotein Binding Proteins (p105/p130) as T-Cadherin and Regulation by Positive and Negative Growth Regulators
| Autor: | Frances Kern, Yelena S. Kuzmenko, Valery N. Bochkov, Dmitry Stambolsky, Thérèse J. Resink, Vsevolod A. Tkachuk |
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| Rok vydání: | 1998 |
| Předmět: |
medicine.medical_specialty
Platelet-derived growth factor Biophysics chemistry.chemical_element Calcium Biochemistry Muscle Smooth Vascular chemistry.chemical_compound Transforming Growth Factor beta Internal medicine medicine Animals Humans Growth Substances Molecular Biology Cells Cultured Platelet-Derived Growth Factor Forskolin biology Cadherin Cell Biology Transforming growth factor beta Cadherins Angiotensin II Molecular biology Growth Inhibitors Rats Lipoproteins LDL Endocrinology chemistry Low-density lipoprotein cardiovascular system biology.protein Carrier Proteins Endothelin receptor Cell Division |
| Zdroj: | Biochemical and Biophysical Research Communications. 246:489-494 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.1998.8645 |
| Popis: | Smooth muscle cells (SMC) express atypical surface low density lipoprotein (LDL) binding proteins of M(r)105 and M(r)130 (p105 and p130) which have been putatively identified as the cell adhesion glycoprotein T-cadherin. Using cultured human and rat aortic SMC and analysis by ligand (LDL)- and immuno-blotting techniques we now confirm identity of p105 and p130 as T-cadherin, as adjudged by sensitivity to PI-PLC cleavage, insensitivity to trypsin degradation in the presence of calcium, and immunoreactivity to anti-T-cadherin peptide antisera. The function of T-cadherin (p105/p130) in the vasculature is unknown. The proteins were downmodulated by the peptide growth factors PDGF-BB, IGF, EGF, and bFGF, but not by vasoactive peptide hormones (angiotensin II, vasopressin, bradykinin, and endothelin). TGF beta, a recognized inhibitor of SMC proliferation, per se had no effect but inhibited growth factor-induced p105/p130 downmodulation. Expression of p105/p130 in quiescent SMC and growth-stimulated SMC (respectively, in serum-free and serum or PDGF-BB containing culture conditions) was increased by forskolin and 8-Br-cyclic GMP, both anti-mitogenic substances, but was unaffected by phorbol ester, calcium ionophores, or calcium antagonists. The findings are compatible with a function for the lipoprotein binding proteins (T-cadherin) in negative regulation of SMC growth. |
| Databáze: | OpenAIRE |
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