NS1′ of Flaviviruses in the Japanese Encephalitis Virus Serogroup Is a Product of Ribosomal Frameshifting and Plays a Role in Viral Neuroinvasiveness
| Autor: | Andrew E. Firth, Ezequiel Balmori Melian, Alexander A. Khromykh, Amanda Nouwens, Bradley J. Blitvich, John F. Atkins, Roy A. Hall, J. Leung, Norma M. Wills, Tomoko Nagasaki, Edward Hinzman, Anneke Funk |
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| Rok vydání: | 2010 |
| Předmět: |
viruses
Molecular Sequence Data Immunology Viral Nonstructural Proteins Microbiology Mass Spectrometry Ribosomal frameshift Virus Viral life cycle Virology medicine Amino Acid Sequence Gene Encephalitis Virus Japanese Translational frameshift Base Sequence biology Frameshifting Ribosomal virus diseases biochemical phenomena metabolism and nutrition Japanese encephalitis biology.organism_classification medicine.disease Virus-Cell Interactions Flavivirus Viral replication Insect Science Nucleic Acid Conformation RNA Viral |
| Zdroj: | Journal of Virology. 84:1641-1647 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | Flavivirus NS1 is a nonstructural protein involved in virus replication and regulation of the innate immune response. Interestingly, a larger NS1-related protein, NS1′, is often detected during infection with the members of the Japanese encephalitis virus serogroup of flaviviruses. However, how NS1′ is made and what role it performs in the viral life cycle have not been determined. Here we provide experimental evidence that NS1′ is the product of a −1 ribosomal frameshift event that occurs at a conserved slippery heptanucleotide motif located near the beginning of the NS2A gene and is stimulated by a downstream RNA pseudoknot structure. Using site-directed mutagenesis of these sequence elements in an infectious clone of the Kunjin subtype of West Nile virus, we demonstrate that NS1′ plays a role in viral neuroinvasiveness. |
| Databáze: | OpenAIRE |
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