Column-switching techniques for high-performance liquid chromatography of ibuprofen and mefenamic acid in human serum with short-wavelength ultraviolet detection
| Autor: | Takatsuka Yashiki, Michio Motohashi, Kenji Yamashita |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Detection limit
Analyte Chromatography Mefenamic acid Chemistry Extraction (chemistry) Ibuprofen General Chemistry Hydrogen-Ion Concentration medicine.disease_cause High-performance liquid chromatography Mefenamic Acid medicine Humans Spectrophotometry Ultraviolet Selectivity Ultraviolet Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of chromatography. 570(2) |
| Popis: | Column-switching techniques for high-performance liquid chromatography of two acidic drugs, ibuprofen and mefenamic acid, in human serum with short-wavelength ultraviolet detection are described. The method involved extraction of the analyte from acidified serum followed by the chromatographic analysis using column switching. Three ODS columns were used each with different mobile phase, utilizing the difference of ion-pair formation or of ionization caused by pH change. The method offered high sensitivity and selectivity, with short-wavelength ultraviolet detection at 221 nm for ibuprofen and at 219 nm for mefenamic acid. The detection limits were 0.5 ng/ml (2.4 pmol/ml) for ibuprofen and 0.1 ng/ml (0.4 pmol/ml) for mefenamic acid using 1 ml of serum, both at a signal-to-noise ratio of 3. With some modifications, the principle of the method would be applicable to other acidic compounds in biological fluids. |
| Databáze: | OpenAIRE |
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