Thiourethane-functionalized fillers: biological properties and degradation resistance
| Autor: | Carmem S. Pfeifer, Amanda Barreto Ramos, Karinne Bueno Antunes, Luis Felipe Jochims Schneider, Daniela Costa Silva, Larissa Maria Cavalcante, Gutemberg Gomes Alves |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Neutral red
Infrared spectroscopy Composite Resins 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Anti-Infective Agents Materials Testing Humans General Materials Science 030212 general & internal medicine Crystal violet Solubility Cytotoxicity Chemistry Biofilm Water RK1-715 030206 dentistry Antimicrobial Anti-Bacterial Agents Dentistry Degradation (geology) Biomarkers Nuclear chemistry |
| Zdroj: | Brazilian Oral Research v.35 2021 Brazilian Oral Research Sociedade Brasileira de Pesquisa Odontológica (SBPqO) instacron:SBPQO Brazilian Oral Research, Volume: 35, Article number: e018, Published: 18 DEC 2020 |
| Popis: | This study determined the effect of thiourethane-functionalized fillers (TU) on the antimicrobial properties, cytotoxicity, degree of conversion (DC), water sorption (Wsp) and solubility (Wsl) of experimental composites. TU-modified fillers were added at different ratios in experimental composites: 0 (Control-TU0), 25% (TU25), 50% (TU50), 75% (TU75) and 100wt% (TU100). The antimicrobial properties were detected through the exhaustion test and counting of Streptococus mutans colonies for biofilm formation. Cytotoxicity to human gingival fibroblasts was evaluated in three different parameters: XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide), NRU (Neutral Red Uptake assay) and CVDE (Crystal Violet Dye Exclusion test)) at the same cells. ELISA was used to measure the IL-6 and b-FGF biomarkers. DC was determined by Fourier-transformed infrared spectroscopy, while Wsp and Wsl by mass variations. Inhibitory capacity of biofilm formation was not observed for any material. All groups presented at least 70% of cell survival within the observed periods (24h and 7 days). Positive control (toxic) had high IL-6 values and low b-FGF values. No significant variations in DC, Wsp, and Wsl were observed among the experimental groups. The use of thiourethane did not present antimicrobial and cytotoxic activity and the tested materials presented equivalent properties to those conventionally used in dentistry. |
| Databáze: | OpenAIRE |
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