In vitro culture of hematopoietic stem cell niche using angiopoietin-1-coupled alginate hydrogel

Autor:	Jae Won Lee, Hyun Seung Kim, Soo-Jeong Yon, Takuya Matsumoto, Sang-Kyung Lee, Kuen Yong Lee
Rok vydání:	2021
Předmět:	Mice Structural Biology Alginates Angiopoietin-1 Animals Hydrogels General Medicine Stem Cell Niche Hematopoietic Stem Cells Molecular Biology Biochemistry
Zdroj:	International journal of biological macromolecules. 209
ISSN:	1879-0003
Popis:	Stem cells exist and maintain their quiescence and pluripotency in stem cell niche. Here, we hypothesized that regulation of cell-cell interactions using a polymeric scaffold as synthetic extracellular matrix (ECM) could be critical in creating a hematopoietic stem cell (HSC) niche in vitro. Angiopoietin-1 (Ang1) binds to the tyrosine kinase receptor (Tie2), and regulation of the Tie2/Ang1 interaction is important in maintaining the quiescence of HSCs in vivo. Alginate hydrogel was thus modified with Ang1 as a synthetic ECM to mimic the HSC niche. Long-term HSCs (CD34
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e55ff17041f3a52b6c362cb771166cb5 https://pubmed.ncbi.nlm.nih.gov/35489624 Zobrazit plný text záznamu Full Text from ScienceDirect