Disparate E-cadherin mutations in LCIS and associated invasive breast carcinomas
| Autor: | John M. Dugan, John A. Pezza, Summerhayes Ic, Kevin S. Hughes, Kimberly M. Rieger-Christ, Braasch Jw |
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| Rok vydání: | 2001 |
| Předmět: |
Paper
Pathology medicine.medical_specialty Lobular carcinoma Mammary gland Breast Neoplasms Biology Pathology and Forensic Medicine Breast cancer medicine Humans Neoplasm Invasiveness skin and connective tissue diseases Polymorphism Single-Stranded Conformational beta Catenin Microdissection Carcinoma in situ Ductal carcinoma Cadherins medicine.disease Neoplasm Proteins Carcinoma Lobular Cytoskeletal Proteins medicine.anatomical_structure Tumor progression Mutation Disease Progression Trans-Activators Immunohistochemistry Female Carcinoma in Situ |
| Zdroj: | Molecular Pathology. 54:91-97 |
| ISSN: | 1366-8714 |
| DOI: | 10.1136/mp.54.2.91 |
| Popis: | Aims—The relation between lobular carcinoma in situ (LCIS) and invasive breast cancer is unresolved. In an attempt to establish whether LCIS is a precursor of invasive cancer the mutational status and the expression of E-cadherin was analysed in LCIS and associated invasive breast carcinoma in 23 patients. Methods—Foci of LCIS and associated invasive carcinoma were individually microdissected from tissue from 23 patients. Exons 4–16 of the E-cadherin gene were analysed using single strand conformation polymorphism (SSCP); protein expression and the localisation of E-cadherin and β-catenin were assessed with the use of immunohistochemistry. Results—Immunohistochemistry revealed a lack of expression of E-cadherin and β-catenin in most LCIS samples and invasive foci. In all but four cases, the staining pattern was identical in the LCIS and associated invasive areas. When E-cadherin was absent, β-catenin was also undetected, suggesting a lack of expression of alternative classic cadherin members in these lesions. Coincident E-cadherin mutations in LCIS and associated invasive carcinoma were not identified in this series of patients. However, mutational analysis of E-cadherin in multiple foci of carcinoma in situ surrounding an invasive lesion provided evidence to support ductal carcinoma in situ as a precursor of invasive ductal carcinoma. Conclusion—These data support the hypothesis that LCIS is not a precursor of invasive breast carcinoma but a marker of increased risk of developing invasive disease. |
| Databáze: | OpenAIRE |
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