In silico study of bone tissue regeneration in an idealised porous hydrogel scaffold using a mechano-regulation algorithm

Autor: Ted J. Vaughan, Laoise M. McNamara, Myles J. Mc Garrigle, Feihu Zhao
Přispěvatelé: Orthopaedic Biomechanics
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Scaffold
Materials science
Bone Regeneration
0206 medical engineering
Mechano-regulation algorithm
Bone and Bones/physiology
Apoptosis
02 engineering and technology
Bone tissue
Bone and Bones
In silico bone tissue engineering
Cell Line
Tissue Scaffolds/chemistry
Mice
Tissue engineering
Cell Movement
medicine
Animals
Computer Simulation
Bone regeneration
Cell Proliferation
Mechanical stimulation
Tissue Scaffolds
Mechanical Engineering
Cartilage
Regeneration (biology)
Biomaterial
Hydrogels
Cell Differentiation
Fibroblasts
021001 nanoscience & nanotechnology
Chondrogenesis
Hydrogels/chemistry
020601 biomedical engineering
Biomechanical Phenomena
medicine.anatomical_structure
Phenotype
Fibroblasts/cytology
Modeling and Simulation
0210 nano-technology
Porosity
Algorithms
Biotechnology
Biomedical engineering
Zdroj: Biomechanics and Modeling in Mechanobiology, 17(1), 5-18. Springer
ISSN: 1617-7959
Popis: Mechanical stimulation, in the form of fluid perfusion or mechanical strain, enhances osteogenic differentiation and overall bone tissue formation by mesenchymal stems cells cultured in biomaterial scaffolds for tissue engineering applications. In silico techniques can be used to predict the mechanical environment within biomaterial scaffolds, and also the relationship between bone tissue regeneration and mechanical stimulation, and thereby inform conditions for bone tissue engineering experiments. In this study, we investigated bone tissue regeneration in an idealised hydrogel scaffold using a mechano-regulation model capable of predicting tissue differentiation, and specifically compared five loading cases, based on known experimental bioreactor regimes. These models predicted that low levels of mechanical loading, i.e. compression (0.5% strain), pore pressure of 10 kPa and a combination of compression (0.5%) and pore pressure (10 kPa), could induce more osteogenic differentiation and lead to the formation of a higher bone tissue fraction. In contrast greater volumes of cartilage and fibrous tissue fractions were predicted under higher levels of mechanical loading (i.e. compression strain of 5.0% and pore pressure of 100 kPa). The findings in this study may provide important information regarding the appropriate mechanical stimulation for in vitro bone tissue engineering experiments.
Databáze: OpenAIRE
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