Glutathione S-transferases related to P. aeruginosa lung infection in cystic fibrosis children: preliminary study
| Autor: | Bernard Lacour, Isabelle Sermet, Patricia Verrier, Jean-Paul Bonnefont, Mairead Kelly, Gérard Lenoir, M.N. Feuillet-Fieux, Marie-Anne Loriot, Thao Nguyen-Khoa, Philippe de Villartay, Philippe Beaune |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Erythrocytes Adolescent Cystic Fibrosis Genotype medicine.medical_treatment Clinical Biochemistry Ascorbic Acid medicine.disease_cause Cystic fibrosis Polymerase Chain Reaction GSTP1 chemistry.chemical_compound Young Adult medicine Humans Vitamin E Pseudomonas Infections Child Vitamin A Chromatography High Pressure Liquid Glutathione Transferase biology Pseudomonas aeruginosa General Medicine Glutathione Ascorbic acid medicine.disease Glutathione S-transferase chemistry Child Preschool Immunology biology.protein Female Polymorphism Restriction Fragment Length |
| Zdroj: | Clinical biochemistry. 42(1-2) |
| ISSN: | 1873-2933 |
| Popis: | Objectives In cystic fibrosis (CF) children, we investigated the predictive impact of glutathione S-transferases (GST) activity and genotypes P1, M1 and T1, and antioxidant levels on stage-severity of Pseudomonas aeruginosa lung infection. Methods GST activity was determined in whole blood by spectrophotometry, and GST genotypes by multiplex PCR RFLP for 36 CF and 9 control children. Levels of glutathione in erythrocyte and vitamins A, E and C in plasma were measured by HPLC. Results No difference in GST activity and no relationship between GST activity and antioxidant levels were observed in CF children as compared to controls. However, GST activity was lower in CF children with severe clinical status and infection, and the frequency of GSTP1 wild type genotype AA, prevalent in uninfected CF children (75%), decreased in infected ones (33%). Conclusion GST activity and genotype could play an important role in modulating P. aeruginosa lung infection in CF patients. |
| Databáze: | OpenAIRE |
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