Voluntary Chronic Heavy Alcohol Consumption in Male Rhesus Macaques Suppresses Cancellous Bone Formation and Increases Bone Marrow Adiposity
Autor: | Adam J. Branscum, Nicole A.R. Walter, Gianni F. Maddalozzo, David B. Burr, Russell T. Turner, Natali Newman, Arianna M. Kahler-Quesada, Urszula T. Iwaniec, Kathleen A. Grant, Matthew R. Allen |
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Rok vydání: | 2019 |
Předmět: |
Male
medicine.medical_specialty Alcohol Drinking Osteocalcin 030508 substance abuse Medicine (miscellaneous) Lumbar vertebrae Toxicology Article Bone remodeling 03 medical and health sciences 0302 clinical medicine Bone Density Bone Marrow Internal medicine medicine Animals Adiposity Bone mineral Lumbar Vertebrae biology Ethanol business.industry Osteoblast Bone fracture medicine.disease Macaca mulatta Psychiatry and Mental health medicine.anatomical_structure Endocrinology Cancellous Bone biology.protein Bone marrow Collagen 0305 other medical science business Cancellous bone 030217 neurology & neurosurgery |
Zdroj: | Alcohol Clin Exp Res |
ISSN: | 1530-0277 |
Popis: | Background Chronic heavy alcohol consumption is an established risk factor for bone fracture, but comorbidities associated with alcohol intake may contribute to increased fracture rates in alcohol abusers. To address the specific effects of alcohol on bone, we used a nonhuman primate model and evaluated voluntary alcohol consumption on: (i) global markers of bone turnover in blood and (ii) cancellous bone mass, density, microarchitecture, turnover, and microdamage in lumbar vertebra. Methods Following a 4-month induction period, 6-year-old male rhesus macaques (Macaca mulatta, n = 13) voluntarily self-administered water or ethanol (EtOH; 4% w/v) for 22 h/d, 7 d/wk, for a total of 12 months. Control animals (n = 9) consumed an isocaloric maltose-dextrin solution. Tetracycline hydrochloride was administered orally 17 and 3 days prior to sacrifice to label mineralizing bone surfaces. Global skeletal response to EtOH was evaluated by measuring plasma osteocalcin and carboxyterminal collagen cross-links (CTX). Local response was evaluated in lumbar vertebra using dual-energy X-ray absorptiometry, microcomputed tomography, static and dynamic histomorphometry, and histological assessment of microdamage. Results Monkeys in the EtOH group consumed an average of 2.8 ± 0.2 (mean ± SE) g/kg/d of EtOH (30 ± 2% of total calories), resulting in an average blood EtOH concentration of 88.3 ± 8.8 mg/dl 7 hours after the session onset. Plasma CTX and osteocalcin tended to be lower in EtOH-consuming monkeys compared to controls. Significant differences in bone mineral density in lumbar vertebrae 1 to 4 were not detected with treatment. However, cancellous bone volume fraction (in cores biopsied from the central region of the third vertebral body) was lower in EtOH-consuming monkeys compared to controls. Furthermore, EtOH-consuming monkeys had lower osteoblast perimeter and mineralizing perimeter, no significant difference in osteoclast perimeter, and higher bone marrow adiposity than controls. No significant differences between groups were detected in microcrack density (2nd lumbar vertebra). Conclusions Voluntary chronic heavy EtOH consumption reduces cancellous bone formation in lumbar vertebra by decreasing osteoblast-lined bone perimeter, a response associated with an increase in bone marrow adiposity. |
Databáze: | OpenAIRE |
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