A Versatile Negative-Staining Ribonuclease Zymogram
Autor: | Claudi M. Cuchillo, E. Fernández, Marc Ribó, J. Bravo, R. de Llorens |
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Rok vydání: | 1994 |
Předmět: |
Biophysics
Adenocarcinoma Bovine pancreatic ribonuclease Sensitivity and Specificity Biochemistry Cell Line Ribonucleases Tumor Cells Cultured Animals Humans Ribonuclease Molecular Biology Polyacrylamide gel electrophoresis Gel electrophoresis Chromatography Staining and Labeling biology Chemistry Ribonuclease Pancreatic Cell Biology Negative stain Staining Pancreatic Neoplasms Electrophoresis Poly C biology.protein Silver Nitrate Cattle Electrophoresis Polyacrylamide Gel Pancreatic ribonuclease |
Zdroj: | Analytical Biochemistry. 219:82-86 |
ISSN: | 0003-2697 |
DOI: | 10.1006/abio.1994.1234 |
Popis: | A versatile negative-staining ribonuclease zymogram is described. The method has several advantages as it combines, by means of different staining procedures, high resolving power, sensitivity, and specificity with a rapid, reproducible, and simultaneous analysis of purity of ribonuclease samples on the same polyacrylamide gel. Activity bands can be visualized at any time during the incubation process without staining of the gel. This allows the choice of different staining procedures after incubation. Using poly(C) as substrate less than 1 pg of bovine pancreatic ribonuclease A was detected in less than 2 h after the electrophoretic run. An additional advantage with respect to other methods is that no refrigeration is needed during electrophoresis. |
Databáze: | OpenAIRE |
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