Single cell analysis reveals the involvement of the long non-coding RNA Pvt1 in the modulation of muscle atrophy and mitochondrial network
| Autor: | Gerolamo Lanfranchi, Stefano Cagnin, Maria Lina Massimino, Chiara Romualdi, Beniamina Pacchioni, Francesco Chemello, Paolo Martini, Caterina Millino, Alessandro Bertoli, Luca Scorrano, Enrico Alessio, Caterina Peggion, Lisa Buson, Francesca Grespi |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Cell
Apoptosis Data Resources and Analyses Mitochondrion Biology Mice 03 medical and health sciences 0302 clinical medicine Single-cell analysis atrophy Genetics medicine Animals Humans Myocyte skeletal muscle Muscle Skeletal In Situ Hybridization Fluorescence 030304 developmental biology 0303 health sciences long non-coding RNA Genome Human Gene Expression Profiling Autophagy Mitophagy Skeletal muscle long non-coding RNA skeletal muscle mitochondria atrophy Muscle atrophy Long non-coding RNA Cell Compartmentation Cell biology mitochondria Muscular Atrophy medicine.anatomical_structure Female RNA Long Noncoding Single-Cell Analysis medicine.symptom 030217 neurology & neurosurgery Muscle Contraction |
| Zdroj: | Nucleic Acids Research |
| Popis: | Long non-coding RNAs (lncRNAs) are emerging as important players in the regulation of several aspects of cellular biology. For a better comprehension of their function, it is fundamental to determine their tissue or cell specificity and to identify their subcellular localization. In fact, the activity of lncRNAs may vary according to cell and tissue specificity and subcellular compartmentalization. Myofibers are the smallest complete contractile system of skeletal muscle influencing its contraction velocity and metabolism. How lncRNAs are expressed in different myofibers, participate in metabolism regulation and muscle atrophy or how they are compartmentalized within a single myofiber is still unknown. We compiled a comprehensive catalog of lncRNAs expressed in skeletal muscle, associating the fiber-type specificity and subcellular location to each of them, and demonstrating that many lncRNAs can be involved in the biological processes de-regulated during muscle atrophy. We demonstrated that the lncRNA Pvt1, activated early during muscle atrophy, impacts mitochondrial respiration and morphology and affects mito/autophagy, apoptosis and myofiber size in vivo. This work corroborates the importance of lncRNAs in the regulation of metabolism and neuromuscular pathologies and offers a valuable resource to study the metabolism in single cells characterized by pronounced plasticity. |
| Databáze: | OpenAIRE |
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