Preparation of chitosan particles suitable for enzyme immobilization
| Autor: | János Gyenis, Tivadar Feczkó, Emese Biro, Ágnes S. Németh, Csaba Sisak |
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| Rok vydání: | 2008 |
| Předmět: |
Chitosan
Aqueous solution Immobilized enzyme Chemistry Precipitation (chemistry) Biophysics Nanoparticle Enzymes Immobilized beta-Galactosidase Biochemistry Catalysis Enzyme Activation Surface-Active Agents chemistry.chemical_compound Chemical engineering Polymer chemistry Emulsion Microscopy Electron Scanning Nanoparticles Particle Particle size Particle Size |
| Zdroj: | Journal of Biochemical and Biophysical Methods. 70:1240-1246 |
| ISSN: | 0165-022X |
| DOI: | 10.1016/j.jprot.2007.11.005 |
| Popis: | Macro-, micro- and nanosized chitosan particles suitable as immobilization carriers were prepared by precipitation, emulsion cross-linking and ionic gelation methods, respectively. Effects of particle preparation parameters on particle size were investigated. Activities of beta-galactosidase covalently attached to differently sized particles have been evaluated and compared. The highest activity was shown by the biocatalyst immobilized on nanoparticles obtained by means of the ionotropic gelation method with sodium sulphate as gelation agent. beta-Galactosidase fixed on macro- and microspheres exhibited excellent storage stability in aqueous solution, with no more than 5% loss of activity after 3 weeks storage at 4 degrees C and pH 7.0. |
| Databáze: | OpenAIRE |
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