Difluoromethylornithine inhibits hypertrophic, pro-fibrotic and pro-apoptotic actions of aldosterone in cardiac cells
| Autor: | Flavio Flamigni, Annalisa Facchini, Carla Pignatti, Ivana Stanic, Silvia Cetrullo, Benedetta Tantini, Claudio Marcello Caldarera |
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| Přispěvatelé: | Cetrullo S., Facchini A., Stanic I., Tantini B., Pignatti C., Caldarera C.M., Flamigni F. |
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Eflornithine Heart Diseases Clinical Biochemistry CARDIAC CELLS Gene Expression Apoptosis Biology Biochemistry Muscle hypertrophy chemistry.chemical_compound Fibrosis Internal medicine medicine Animals Myocytes Cardiac Rats Wistar Gene Aldosterone Cells Cultured chemistry.chemical_classification Organic Chemistry Biogenic Polyamines Hypertrophy Ornithine medicine.disease POLYAMINES Rats Enzyme Endocrinology chemistry Heart failure DIFLUOROMETHYLORNITHINE |
| Zdroj: | Amino acids. 38(2) |
| ISSN: | 1438-2199 |
| Popis: | Recent studies have shown that aldosterone may play a critical role in the transition to heart failure and that heart is a direct target of the action of aldosterone, which can provoke hypertrophy and apoptosis of isolated cardiomyocytes and also increase the expression of genes that favor tissue fibrosis. Early work from this and other laboratories has established a link between the aliphatic polyamines and cardiac hypertrophy, while more recently an involvement of polyamines even in cell death and survival has emerged. In the present study we have treated cardiac cells, i.e. rat H9c2 cardiomyoblasts and neonatal cardiomyocytes, with (D, L)-2-(difluoromethyl)ornithine, a specific inhibitor of polyamine biosynthesis, to investigate the effects of polyamines in relation to the hypertrophic, pro-fibrotic and pro-apoptotic actions of aldosterone. The results indicate that inhibition of polyamine biosynthesis may prevent or attenuate the adverse actions of aldosterone, by modulating the expression of genes related to cardiac hypertrophy and fibrosis, as well as the levels of proteins and the activities of enzymes that control apoptosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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