MKP-1 Modulates Mitochondrial Transcription Factors, Oxidative Phosphorylation, and Glycolysis
| Autor: | Christian Bauerfeld, Harvinder Talwar, Lobelia Samavati, Yusen Liu, Kezhong Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Immunology
Oxidative phosphorylation p38 Mitogen-Activated Protein Kinases Article Oxidative Phosphorylation Mice Sepsis Immunology and Allergy Animals Glycolysis Transcription factor Organelle Biogenesis Chemistry Nuclear Respiratory Factor 1 Macrophages High Mobility Group Proteins Dual Specificity Phosphatase 1 General Medicine TFAM Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha Cell biology Mitochondria DNA-Binding Proteins Mitochondrial biogenesis Anaerobic glycolysis MAPK phosphatase Organelle biogenesis Reactive Oxygen Species Transcription Factors |
| Zdroj: | ImmunoHorizons |
| ISSN: | 2573-7732 |
| Popis: | Sepsis is the leading cause of death in the world. Recent reports suggest that in response to sepsis, metabolism of macrophages switches from oxidative phosphorylation to aerobic glycolysis. MAPK phosphatase (MKP)–1 (also known as DUSP1) localized in the nucleus and preferentially dephosphorylates p38 and JNK. MKP-1 controls the expression of numerous inflammatory genes and transcription factors, thereby regulating innate and adaptive immunity. MKP-1–deficient animals exhibit aberrant metabolic responses following bacterial infections with a markedly increased mortality in response to sepsis. Because metabolic reprogramming modulates immune responses to TLR-4 activation, we investigated the effect of MKP-1 deficiency on mitochondrial electron transport chains involved in oxidative phosphorylation and transcription factors regulating mitochondrial biogenesis. Mitochondrial biogenesis is regulated by three nuclear-encoded proteins, including transcription factor A (TFAM), nuclear respiratory factors (NRF-1), and peroxisome proliferator–activated receptor γ coactivator-1-α (PGC-1α). We show that MKP-1–deficient mice/macrophages exhibit, at baseline, higher expression of oxidative phosphorylation, TFAM, PGC-1α, and NRF-1 associated with increased respiration and production of reactive oxygen species as compared with wild-type mice. Surprisingly, MKP-1–deficient mice/macrophages responded to Escherichia coli sepsis or LPS with an impaired metabolic switch; despite enhanced glycolysis, a preserved mitochondrial function and biogenesis are exhibited. Furthermore, inhibition of p38 MAPK had no significant effect on TFAM and NRF-1 either in MKP-1–deficient macrophages or in wild-type macrophages. These findings support the conclusion that MKP-1 plays an important role in regulating proteins involved in glycolysis and oxidative phosphorylation and modulates expression of mitochondrial transcription factors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|