A Comparison of Exogenous Labels for the Histological Identification of Transplanted Neural Stem Cells
| Autor: | Jessie R. Liu, Michel Modo, Francesca J. Nicholls |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Quality Control 0301 basic medicine Cell Survival Population Cell Biomedical Engineering lcsh:Medicine Context (language use) Biology Article Rats Sprague-Dawley Andrology 03 medical and health sciences 0302 clinical medicine Neural Stem Cells Antigen In vivo medicine Animals Humans education Cell Proliferation Transplantation education.field_of_study Staining and Labeling lcsh:R Cell Biology Immunohistochemistry In vitro Neural stem cell 030104 developmental biology medicine.anatomical_structure 030217 neurology & neurosurgery |
| Zdroj: | Cell Transplantation, Vol 26 (2017) |
| ISSN: | 1555-3892 0963-6897 |
| Popis: | The interpretation of cell transplantation experiments is often dependent on the presence of an exogenous label for the identification of implanted cells. The exogenous labels Hoechst 33342, 5-bromo-2′-deoxyuridine (BrdU), PKH26, and Qtracker were compared for their labeling efficiency, cellular effects, and reliability to identify a human neural stem cell (hNSC) line implanted intracerebrally into the rat brain. Hoechst 33342 (2 mg/ml) exhibited a delayed cytotoxicity that killed all cells within 7 days. This label was hence not progressed to in vivo studies. PKH26 (5 μM), Qtracker (15 nM), and BrdU (0.2 μM) labeled 100% of the cell population at day 1, although BrdU labeling declined by day 7. BrdU and Qtracker exerted effects on proliferation and differentiation. PKH26 reduced viability and proliferation at day 1, but this normalized by day 7. In an in vitro coculture assay, all labels transferred to unlabeled cells. After transplantation, the reliability of exogenous labels was assessed against the gold standard of a human-specific nuclear antigen (HNA) antibody. BrdU, PKH26, and Qtracker resulted in a very small proportion ( |
| Databáze: | OpenAIRE |
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