Seroreactive recombinant herpes simplex virus type 2-specific glycoprotein G
| Autor: | J M Rose, D. L. Parkes, S. R. Coates, C. M. Smith, J. W. Brandis |
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| Rok vydání: | 1991 |
| Předmět: |
Adult
Microbiology (medical) Genes Viral medicine.drug_class viruses Gene Expression Antibodies Viral Monoclonal antibody Epitope law.invention Viral Envelope Proteins law Protein A/G Escherichia coli medicine Humans Simplexvirus Cloning Molecular Promoter Regions Genetic Antiserum biology Antibodies Monoclonal Herpes Simplex Bacteriophage lambda Virology Molecular biology Fusion protein Recombinant Proteins Molecular Weight biology.protein Recombinant DNA Protein G Research Article Myc-tag |
| Zdroj: | Journal of Clinical Microbiology. 29:778-781 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | The herpes simplex virus type 2 (HSV-2) genome codes for an envelope protein, glycoprotein G (gG), which contains predominantly type 2-specific epitopes. A portion of this gG gene has been expressed as a fusion protein in Escherichia coli. Expression was regulated by a lambda phage pL promoter. The 60,000-molecular-weight recombinant protein was purified by ion-exchange chromatography. Amino acid sequence analysis confirmed the N terminus of the purified protein. Mice immunized with recombinant gG developed antibodies reactive with native HSV-2 protein, but not with HSV-1 protein, in an indirect immunofluorescence assay. The serological activity of this purified recombinant gG protein was evaluated by immunoblot assay. This protein was reactive with an HSV-2 gG monoclonal antibody. It was also reactive with HSV-2 rabbit antiserum but not with HSV-1 rabbit antiserum. Of 15 patient serum samples known to have antibody to HSV-2, 14 were reactive with this recombinant type 2-specific gG protein, and none of 15 HSV antibody-negative patient serum samples showed reactivity. In agreement with the expected prevalence of HSV-2 infection, 27.6% of 134 serum samples from random normal individuals had antibodies reactive with recombinant gG. This recombinant gG protein may be of value in detecting HSV-2-specific antibody responses in patients infected with HSV-2. |
| Databáze: | OpenAIRE |
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