Lack of effect of 50-Hz magnetic field exposure on the binding affinity of serotonin for the 5-HT 1B receptor subtype
| Autor: | Florence Poulletier de Gannes, Hiroshi Masuda, Bernard Billaudel, Bernard Veyret, Emmanuelle Haro, Gilles Ruffié, Isabelle Lagroye |
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| Přispěvatelé: | Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Bioélectromagnétisme, École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Agonist
Serotonin medicine.medical_specialty medicine.drug_class Biology Inhibitory postsynaptic potential 03 medical and health sciences chemistry.chemical_compound Electromagnetic Fields 0302 clinical medicine Internal medicine Cyclic AMP medicine Animals Neurotransmitter Receptor Molecular Biology Cells Cultured 5-HT receptor 030304 developmental biology 0303 health sciences General Neuroscience Cell Membrane Colforsin Brain Transfection Tryptamines Rats [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biophysics Endocrinology chemistry [SDV.TOX]Life Sciences [q-bio]/Toxicology Receptor Serotonin 5-HT1B Neurology (clinical) Signal transduction Neuroglia 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Brain Research Brain Research, Elsevier, 2011, 1368, pp.44-51. ⟨10.1016/j.brainres.2010.10.103⟩ |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2010.10.103⟩ |
| Popis: | International audience; There is some concern that exposure to extremely low-frequency magnetic fields (MF) causes adverse health effects via signal transduction pathways. Two previous studies reported that exposure to 50-Hz MF decreased the binding affinity of the 1B receptor subtype of serotonin (5-HT) in rat brain membranes. The aim of this study was to investigate whether the exposure to MF affects binding to the 5-HT(1B) receptor and a physiological function associated with 5-HT(1B) receptor activation. Rat brain crude membrane fractions, including 5-HT(1B) receptor and C6-glial cells transfected with human 5-HT(1B) receptor gene, were exposed to 50-Hz MF at 1 mT using Merritt coils under temperature-regulated conditions. In the rat crude membrane, there was no significant difference in the affinity constant of [(3)H]-5-HT between exposed (K(d): 0.92±0.38 nM) and sham-exposed (K(d): 1.00±0.32 nM). The lack of affinity change after exposure was also confirmed using a chemical agonist of the 5-HT receptor, [(3)H]-5-carboxytryptamine (K(d): 0.59±0.06 nM for exposed and 0.71±0.08 nM for sham). Similar negative results in terms of affinity constant were obtained on the human 5-HT(1B) receptor in C6-glial cells. In addition, forskolin-stimulated cAMP production was inhibited by 5-HT administration in a dose-dependent manner in C6-glial cells, but exposure did not modify the inhibitory response. This study thus failed to confirm the previous results and findings suggest that exposure to MF below the current occupational limit does not affect the physiological function involved in 5-HT(1B) receptor subtypes. |
| Databáze: | OpenAIRE |
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