Assessment of food microbiological indicators applied on poultry carcasses by culture combined MALDI-TOF MS identification and 16S rRNA amplicon sequencing
| Autor: | Kurt Houf, Marie Joossens, Charles Dumolin, Zhongjia Yu, Maria Francesca Peruzy |
|---|---|
| Přispěvatelé: | Yu, Zhongjia, Peruzy, Maria Francesca, Dumolin, Charle, Joossens, Marie, Houf, Kurt |
| Rok vydání: | 2019 |
| Předmět: |
DNA
Bacterial Meat Aerobic bacteria Staphylococcus Biology medicine.disease_cause 16S rRNA amplicon sequencing Microbiology Poultry 03 medical and health sciences chemistry.chemical_compound Chicken meat Pseudomonas RNA Ribosomal 16S Escherichia coli medicine MALDI-TOF MS Animals Food science Phylogeny 030304 developmental biology 0303 health sciences ISO method Bacteria 030306 microbiology Genetic Variation Sequence Analysis DNA MRS agar 16S ribosomal RNA biology.organism_classification DNA extraction Bacterial Typing Techniques Bacterial diversity chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Food Microbiology Food Science |
| Zdroj: | Food Microbiology. 82:53-61 |
| ISSN: | 0740-0020 |
| DOI: | 10.1016/j.fm.2019.01.018 |
| Popis: | Examination of the bacterial contamination on food products is still largely performed by standardized culture methods, though culture-independent methods are suggested as a more reliable approach. Knowledge of the diversity of bacteria isolated from food as well as the impact of the plate incubation conditions applied are still understudied. The impact of incubation at 7 °C and 30 °C on total aerobic bacterial count and diversity, and the performance of ISO methods generally applied in microbiological quality examination were assessed by culture combined MALDI-TOF MS identification and 16S rRNA amplicon sequencing. Examining breast skin of 16 chicken carcasses, no significant impact of the incubation temperature on the total aerobic bacteria level and diversity was detected, limiting the usefulness of additional psychrophilic examination. Bacteria phenotypically similar to Pseudomonas, were identified on selective CFC plates, and on MRS agar plates for lactic acid bacteria, Escherichia coli and Staphylococcus were commonly present. Application of 16S rRNA amplicon sequencing revealed a higher bacterial diversity, but the impact of the DNA extraction kit applied, and the detection of non-viable bacteria should be taken into account to interpret the final outcome. |
| Databáze: | OpenAIRE |
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