Potential of temperature- and salinity-driven shifts in diatom compatible solute concentrations to impact biogeochemical cycling within sea ice
| Autor: | Angela K. Boysen, Jodi N. Young, Hannah M. Dawson, Katherine R. Heal, Laura T. Carlson, Anitra E. Ingalls |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Atmospheric Science Biogeochemical cycle Environmental Engineering cryoprotection Sea ice Metabolomics Diatom Osmoprotection Cryoprotection Metabolism 030106 microbiology Temperature salinity diagrams Oceanography Dimethylsulfoniopropionate osmoprotection 03 medical and health sciences chemistry.chemical_compound Algae 14. Life underwater lcsh:Environmental sciences lcsh:GE1-350 geography geography.geographical_feature_category Ecology biology Chemistry Geology Geotechnical Engineering and Engineering Geology biology.organism_classification metabolomics sea ice diatom Salinity 030104 developmental biology 13. Climate action Environmental chemistry Osmoprotectant metabolism |
| Zdroj: | Elem Sci Anth; Vol 8 (2020); 25 Elementa: Science of the Anthropocene, Vol 8, Iss 1 (2020) |
| ISSN: | 2325-1026 |
| Popis: | Sea-ice algae are an important source of primary production in polar regions, yet we have limited understanding of their responses to the seasonal cycling of temperature and salinity. Using a targeted liquid chromatography-mass spectrometry-based metabolomics approach, we found that axenic cultures of the Antarctic sea-ice diatom, Nitzschia lecointei, displayed large differences in their metabolomes when grown in a matrix of conditions that included temperatures of –1 and 4°C, and salinities of 32 and 41, despite relatively small changes in growth rate. Temperature exerted a greater effect than salinity on cellular metabolite pool sizes, though the N- or S-containing compatible solutes, 2, 3-dihydroxypropane-1-sulfonate (DHPS), glycine betaine (GBT), dimethylsulfoniopropionate (DMSP), and proline responded strongly to both temperature and salinity, suggesting complexity in their control. We saw the largest (> 4-fold) response to salinity for proline. DHPS, a rarely studied but potential compatible solute, had the highest intracellular concentrations among all compatible solutes of ~85 mM. When comparing the culture findings to natural Arctic sea-ice diatom communities, we found extensive overlap in metabolite profiles, highlighting the relevance of culture-based studies to probe environmental questions. Large changes in sea-ice diatom metabolomes and compatible solutes over a seasonal cycle could be significant components of biogeochemical cycling within sea ice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|