Dichlormethane Extract of the Jelly Ear Mushroom Auricularia auricula-judae (Higher Basidiomycetes) Inhibits Tumor Cell Growth In Vitro
| Autor: | Md. Ahsanur Reza, Man Hee Rhee, Joo-Won Suh, Woo-Sik Jo, Sileshi Belew Yohannes, Dereje Damte, Md. Akil Hossain, Seung-Chun Park, Seung-Jin Lee |
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| Rok vydání: | 2014 |
| Předmět: |
Antioxidant
Cell Survival medicine.medical_treatment Apoptosis Applied Microbiology and Biotechnology Cell Line Tumor Vegetables Drug Discovery medicine Humans Cytotoxicity Cell Proliferation Pharmacology Auricularia auricula-judae biology Plant Extracts Cell growth Chemistry biology.organism_classification Antineoplastic Agents Phytogenic Molecular biology Growth Inhibitors In vitro Biochemistry Cell culture Cancer cell Agaricales |
| Zdroj: | International Journal of Medicinal Mushrooms. 16:37-47 |
| ISSN: | 1521-9437 |
| DOI: | 10.1615/intjmedmushr.v16.i1.40 |
| Popis: | In this study, a dichloromethane fraction (DCMF) from 70% Auricularia auricula-judae ethanol extract showed the highest level of antitumor activity compared to other solvent fractions (ethyl acetate, butanol, and water). The DCMF was found to have more potent antitumor activity against broncheoalveolar cancer (half maximal inhibitory concentration = 57.2 µg/mL) and gastric cancer cells (half maximal inhibitory concentration = 73.2 µg/mL) compared to the other solvent fractions, although all fractions inhibited the proliferation of the tumor cells in a dose-dependent manner. We further analyzed the DCMF composition by gas chromatography-coupled mass spectroscopy. Based on the results of this analysis, an antitumor active component (diazane) was identified in the DCMF. However, we found that diazane alone had a lower level of antitumor activity than the DCMF. These findings indicate that other unknown components of the DCMF also are responsible for the cytotoxic effects of DCMF against tumor cells. Semiquantitative reverse transcription polymerase chain reaction analysis demonstrated that DCMF induced cytotoxicity or tumor cell apoptosis as a result of the downregulation of Bcl-2 expression and p53 overexpression. Taken together, our study results demonstrated that the DCMF may be used as a functional additive for enhancing antioxidant activities and suppressing tumor growth in the body. |
| Databáze: | OpenAIRE |
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