Study of the stress proteins secreted byLeishmania donovaniafter treatment with edelfosine, mitelfosine and ilmofosine, and morphological alterations analyzed by electronic microscopy
| Autor: | S. Azzouz, Antonio Osuna, Anne-Françoise Pétavy, M.E. Sarciron, Mimoun Maache |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
alkyl-lysophospholipids
Veterinary (miscellaneous) morphological alteration Drug Resistance Protozoan Proteins Leishmania donovani Antineoplastic Agents Vacuole Biology World Health Organization lcsh:Infectious and parasitic diseases Microbiology amastigote-like forms chemistry.chemical_compound medicine Animals Humans lcsh:RC109-216 Amastigote Axenic Heat-Shock Proteins Miltefosine Phospholipid Ethers Kinetoplastida biology.organism_classification stress proteins Microscopy Electron Infectious Diseases chemistry Biochemistry electronic microscopy Insect Science Autoradiography Leishmaniasis Visceral Protozoa Animal Science and Zoology Parasitology Edelfosine medicine.drug |
| Zdroj: | Parasite, Vol 16, Iss 3, Pp 215-221 (2009) |
| ISSN: | 1776-1042 1252-607X |
| DOI: | 10.1051/parasite/2009163215 |
| Popis: | We studied the stress proteins induced in protozoa Leishmania donovani after treatment with edelfosine, miltefosine and ilmofosine. We studied the morphological and structural modifications caused in the promastigote forms of the parasite after treatment with the three alkyl-lysophospholipids (ALPs). A resistant strain of L. donovani to miltefosine was obtained and the morphological modifications were observed. The stress proteins induction was studied in promastigote forms and also in amastigote-like forms obtained in vitro. The proteins synthesized with the three alkyl-lysophospholipids were compared to those obtained by heat shock. The axenic amastigote forms synthesized a pattern of different proteins for those observed in the promastigote forms. The morphological alterations were observed under electronic microscopy. The membrane and mitochondria were the organs most affected by the three ALPs. We noted an apparition of vacuoles and vesicles in the treated promastigotes. In the resistant strain, we noted myelin bodies in the treated and untreated parasites. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|