Purification of an antihemorrhagic factor from the serum of the non-venomous snake Dinodon semicarinatus
| Autor: | Kazuo Yonaha, Yoshiharu Kawamura, Yasuhiro Tomihara, Takao Kamura, Seizen Toyama, Masanobu Yamakawa, Masatoshi Nozaki |
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| Rok vydání: | 1988 |
| Předmět: |
Gel electrophoresis
Dinodon semicarinatus Chromatography biology Chemistry Trimeresurus flavoviridis Size-exclusion chromatography Antihemorrhagic Snakes Blood Proteins Toxicology biology.organism_classification people.cause_of_death Molecular biology Chromatography DEAE-Cellulose Electrophoresis Venomous snake Animals Spectrophotometry Ultraviolet people Polyacrylamide gel electrophoresis Chromatography High Pressure Liquid |
| Zdroj: | Toxicon. 26:420-423 |
| ISSN: | 0041-0101 |
| DOI: | 10.1016/0041-0101(88)90013-x |
| Popis: | Y. Tomihara , K. Yonaha , M. Nozaki , M. Yamakawa , Y. Kawamura , T. Kamura and S. Toyama . Purification of an antihemorrhagic factor from the serum of the non-venomous snake Dinodon semicarinatus. Toxicon26, 420 – 423, 1988. — An antihemorrhagic factor was purified from the serum of Dinodon semicarinatus, a non-venomous snake (Akamata), by a series of high performance liquid chromatographies with a TSK gel DEAE-5PW column. The purified antihemorrhagic factor showed a single band on polyacrylamide gel disc electrophoresis and inhibited the hemorrhagic activity of HR1 and HR2, the hemorrhagic factors of Trimeresurus flavoviridis Okinawa. The antihemorrhagic factor was stable from 0° to 60° and at pH values 2.0 – 11.0. The molecular weight of the factor was estimated to be 59,000 and 52,000 by a gel filtration and SDS-disc electrophoresis, respectively, suggesting that it consists of a single subunit, as we also found for the antihemorrhagic factors of the mongoose Herpestes edwardsii. |
| Databáze: | OpenAIRE |
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