Profiling of volatile terpenes from almond (Prunus dulcis) young fruits and characterization of seven terpene synthase genes
| Autor: | Haim Reuveny, Mwafaq Ibdah, Adi Faigenboim, Bhagwat Nawade, Mosaab Yahyaa, Irit Bar-Ya’akov, Doron Holland, Liora Shaltiel-Harpaz, Ori Eisenbach |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Farnesene Acyclic Monoterpenes Monoterpene Cyclohexane Monoterpenes Plant Science Biology Sesquiterpene 01 natural sciences Terpene 03 medical and health sciences chemistry.chemical_compound Linalool Botany Genetics Phylogeny Plant Proteins Nerolidol Volatile Organic Compounds Alkyl and Aryl Transferases Terpenes Computational Biology food and beverages General Medicine Prunus dulcis 030104 developmental biology chemistry Fruit Agronomy and Crop Science Geraniol 010606 plant biology & botany |
| Zdroj: | Plant Science. 287:110187 |
| ISSN: | 0168-9452 |
| DOI: | 10.1016/j.plantsci.2019.110187 |
| Popis: | Almond (Prunus dulcis) is an agricultural and economically important fruit tree from the Rosaceae family used in the food industry. The monoterpenes and sesquiterpenes perform important ecological functions such as insecticidal and antifeedant activities against various insects. The young fruits of the different almond varieties were found to produce considerable amounts of terpene volatiles, including linalool and geraniol. To identify terpene synthases (TPSs) involved in the production of these volatile terpenes, existing genome databases of the Rosaceae were screened for almond genes with significant sequence similarity to other plants TPSs. Bioinformatics analysis led to the identification of seven putative TPSs genes with complete open reading frames. We characterized the enzymes encoded by these seven complementary DNAs: the monoterpene synthases PdTPS1, PdTPS3, PdTPS5, and PdTPS6 belong to the TPS-b clade, which catalyzes the formation of β-phellandrene, geraniol, linalool, and farnesene, respectively. The sesquiterpene synthases PdTPS2 and PdTPS4, which belong to the TPS-a clade mainly catalyze the formation of bergamotene, while another sesquiterpene synthase, PdTPS7, from the TPS-g clade showed nerolidol synthase activity. The qRT-PCR analysis revealed that the various tissues of almond varieties showed differential transcription for all these PdTPSs genes. |
| Databáze: | OpenAIRE |
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