Expression of sheep interleukin 23 (IL23A, alpha subunit p19) in two distinct gastrointestinal diseases

Autor: Anton G. Gossner, Virginia M. Venturina, Anna Peers, Craig A. Watkins, John Hopkins
Rok vydání: 2012
Předmět:
Interleukin-23/biosynthesis
Gastric Lymph Node
Paratuberculosis
Trichostrongyloidiasis/veterinary
Interleukin-23
Trichostrongyloidiasis
Trichostrongyloidea/immunology
Sheep Diseases/genetics
Interleukin 23
Cloning
Molecular
Lymph node
Peptide sequence
Interleukin-23/immunology
Trichostrongyloidiasis/genetics
biology
Mycobacterium avium subsp. paratuberculosis/immunology
Paratuberculosis/immunology
Interleukin
Mycobacterium avium subspecies paratuberculosis
Teladorsagia circumcincta
Mycobacterium avium subsp. paratuberculosis
medicine.anatomical_structure
Sheep Diseases/immunology
Mycobacterium avium subsp. paratuberculosis/genetics
Female
Sheep Diseases/microbiology
Trichostrongyloidiasis/immunology
Interleukin-23/genetics
Paratuberculosis/microbiology
Molecular Sequence Data
Immunology
Sheep Diseases
Trichostrongyloidiasis/parasitology
Microbiology
medicine
Animals
Amino Acid Sequence
Alleles
Sheep
Trichostrongyloidea
Base Sequence
General Veterinary
Sequence Analysis
DNA
medicine.disease
biology.organism_classification
veterinary(all)
Paratuberculosis/genetics
Sheep Diseases/parasitology
Trichostrongyloidea/genetics
Sequence Alignment
Zdroj: Gossner, A G, Venturina, V M, Peers, A, Watkins, C A & Hopkins, J 2012, ' Expression of sheep interleukin 23 (IL23A, alpha subunit p19) in two distinct gastrointestinal diseases ', Veterinary Immunology and Immunopathology, vol. 150, no. 1-2, pp. 118-122 . https://doi.org/10.1016/j.vetimm.2012.08.004
ISSN: 0165-2427
DOI: 10.1016/j.vetimm.2012.08.004
Popis: This paper reports the sequence of sheep interleukin 23A (p19), and shows that it shares 98% identity with bovine IL23A, 85% with human and 76% with mouse IL23A. It also reports the existence of two allelic variants that differ largely within the region encoding the amino terminal polypeptide signal sequence. An optimized RT-qPCR assay was used to quantify IL23A transcripts in sheep infected with two common gastrointestinal pathogens, the intracellular bacterium Mycobacterium avium subspecies paratuberculosis and the parasitic nematode Teladorsagia circumcincta. No differential expression of IL23A was detected in the mesenteric lymph node of sheep with the different pathogenic forms of paratuberculosis, however significantly high levels of IL23A were detected in the ileal mucosa of the paucibacillary form in comparison with the asymptomatic or multibacillary forms. Similarly, significantly high levels were present in the gastric lymph node draining T. circumcincta-infected abomasum in susceptible sheep. High levels of IL23A seem to be associated with lymphocytic infiltration and inflammation in both diseases but not with the macrophage infiltrate of multibacillary paratuberculosis.
Databáze: OpenAIRE
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