Expression of a novel gene FAM43B repressing cell proliferation is regulated by DNA methylation in hepatocellular carcinoma cell lines
| Autor: | Xiao Xu, Ze-Guang Han, Jian Huang, Wen Ming Xing, Bingbing Wan, Ruifang Liu |
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| Rok vydání: | 2011 |
| Předmět: |
Carcinoma
Hepatocellular Clinical Biochemistry Down-Regulation Biology medicine.disease_cause Epigenesis Genetic RNA interference Cell Line Tumor medicine Humans Genes Tumor Suppressor Epigenetics Promoter Regions Genetic Molecular Biology Phylogeny Cell Proliferation Cell Nucleus Gene knockdown Cell growth Cell Cycle Liver Neoplasms Cell Biology General Medicine Methylation Cell cycle DNA Methylation Molecular biology digestive system diseases Gene Expression Regulation Neoplastic Organ Specificity DNA methylation Cancer research RNA Interference Carcinogenesis |
| Zdroj: | Molecular and cellular biochemistry. 354(1-2) |
| ISSN: | 1573-4919 |
| Popis: | The epigenetic dysregulation of tumor suppressor genes plays an important role in many cancers, including hepatocellular carcinoma (HCC). In this study, we identified a new gene, family with sequence similarity 43, member B (FAM43B), based on a previous genome-wide approach. FAM43B was significantly downregulated in 60% (24/40) HCC specimens as compared to non-HCC livers. Enforced FAM43B overexpression could suppress cell growth and colony formation in vitro, and induce cell cycle delay, whereas FAM43B knockdown enhanced cell growth. The expression level of FAM43B was found related to the methylation level of FAM43B promoter in HCC cell lines and HCC specimens. The collective data suggest that the expression of FAM43B was regulated by methylation and the epigenetic silencing of FAM43B could contribute to HCC tumorigenesis by regulating cell proliferation. |
| Databáze: | OpenAIRE |
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