Dose–response curves for analyzing of dicentric chromosomes and chromosome translocations following doses of 1000 mGy or less, based on irradiated peripheral blood samples from five healthy individuals
| Autor: | Kurumi Fujioka, Akira Sakai, Kenji Kamiya, Yu Abe, Yumiko Kurosu, Tomisato Miura, Aki Yanagi, Toshiya Inaba, Naohiro Tsuyama, Mitsuaki A. Yoshida, Risa Ujiie |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Male Health Toxicology and Mutagenesis Chromosomal translocation Biology Giemsa staining Translocation Genetic Giemsa stain 030218 nuclear medicine & medical imaging dose–response curve Young Adult 03 medical and health sciences Dicentric chromosome 0302 clinical medicine Regular Paper centromere-FISH Humans Dosimetry Radiology Nuclear Medicine and imaging Irradiation In Situ Hybridization Fluorescence Chromosome Aberrations Genetics Radiation business.industry Chromosome Dose-Response Relationship Radiation Gold standard (test) dicentric chromosome biological dosimetry Middle Aged Tissue Donors Dose–response relationship Gamma Rays 030220 oncology & carcinogenesis Female Nuclear medicine business chromosome translocation |
| Zdroj: | Journal of Radiation Research |
| ISSN: | 1349-9157 0449-3060 |
| DOI: | 10.1093/jrr/rrx052 |
| Popis: | In terms of biological dosimetry at the time of radiation exposure, the dicentric chromosome (Dic) assay (DCA) is the gold standard for assessing for the acute phase and chromosome translocation (Tr) analysis is the gold standard for assessing the chronic phase. It is desirable to have individual dose–response curves (DRCs) for each laboratory because the analysis criteria differ between laboratories. We constructed the DRCs for radiation dose estimation (with three methods) using peripheral blood (PB) samples from five healthy individuals. Aliquots were irradiated with one of eight gamma-ray doses (0, 10, 20, 50, 100, 200, 500 or 1000 mGy), then cultured for 48 h. The number of chromosome aberrations (CAs) was analyzed by DCA, using Giemsa staining and centromere-fluorescence in situ hybridization (centromere-FISH) and by chromosome painting (chromosome pairs 1, 2 and 4) for Tr analysis. In DCA, there was large variation between individuals in the frequency of Dics formed, and the slopes of the DRCs were different. In Tr analysis, although variation was observed in the frequency of Tr, the slopes of the DRCs were similar after adjusting the background for age. Good correlation between the irradiation dose and the frequency of CAs formed was observed with these three DRCs. However, performing three different biological dosimetry assays simultaneously on PB from five donors nonetheless results in variation in the frequency of CAs formed, especially at doses of 50 mGy or less, highlighting the difficulty of biological dosimetry using these methods. We conclude that it might be difficult to construct universal DRCs. |
| Databáze: | OpenAIRE |
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