Involvement of Ventral Periaqueductal Gray Dopaminergic Neurons in Propofol Anesthesia
| Autor: | Yu Zhang, Jia Li, Fu Shi, Bao Fu, Yi Zhang, Zikun Duan, Tian Yu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
medicine.medical_specialty Glutamic Acid Inhibitory postsynaptic potential Biochemistry Periaqueductal gray Rats Sprague-Dawley Random Allocation 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine 030202 anesthesiology Postsynaptic potential Internal medicine medicine Animals Periaqueductal Gray Propofol gamma-Aminobutyric Acid Chemistry Dopaminergic Neurons Dopaminergic Glutamate receptor General Medicine Rats Electrophysiology Endocrinology Receptors GABA-B nervous system Excitatory postsynaptic potential Anesthetics Intravenous 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Neurochemical Research. 43:838-847 |
| ISSN: | 1573-6903 0364-3190 |
| DOI: | 10.1007/s11064-018-2486-y |
| Popis: | It has been reported that central dopaminergic system is implicated in the mechanism underlying general anesthesia. Whether dopamine (DA) neurons in midbrain ventral periaqueductal gray (vPAG) are involved in general anesthesia and how general anesthetics affect these neurons remain sparsely documented. To determine the role of vPAG DA neurons in propofol-induced anesthesia, we performed microinjection of 6-hydroxydopamine (6-OHDA) into vPAG to damage DA neurons and investigated the alteration in somatosensory electroencephalogram (EEG), as well as the induction and recovery time of propofol anesthesia. Subsequently, we examined the effect of propofol on the electrophysiological activity of DA neurons in vPAG using whole-cell patch clamp. Two weeks after 6-OHDA microinfusion, DA neurons in the vPAG were markedly reduced by 63.6% in the 6-OHDA-treated rats compared with vehicle rats. This lesion significantly shortened the induction time (7.15 ± 3.97 s vs. 11.18 ± 2.83 s, P |
| Databáze: | OpenAIRE |
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