Activity-based protein profiling of rice (Oryza sativa L.) bran serine hydrolases
| Autor: | Ram Rajasekharan, Panneerselvam Vijayaraj, Arun Kumar Vijayakumar, Achintya Kumar Dolui |
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| Rok vydání: | 2020 |
| Předmět: |
Dietary Fiber
0106 biological sciences 0301 basic medicine Hydrolases medicine.medical_treatment Protein Array Analysis lcsh:Medicine Plant Proteins Dietary 01 natural sciences Article Serine 03 medical and health sciences Yeasts medicine Storage protein lcsh:Science chemistry.chemical_classification Serine protease Multidisciplinary Protease Oryza sativa Bran biology lcsh:R Activity-based proteomics food and beverages Molecular Sequence Annotation Oryza Yeast 030104 developmental biology Food Storage Biochemistry chemistry Dietary Supplements biology.protein lcsh:Q Plant sciences Biotechnology 010606 plant biology & botany |
| Zdroj: | Scientific Reports, Vol 10, Iss 1, Pp 1-15 (2020) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-020-72002-w |
| Popis: | Rice bran is an underutilized agricultural by-product with economic importance. The unique phytochemicals and fatty acid compositions of bran have been targeted for nutraceutical development. The endogenous lipases and hydrolases are responsible for the rapid deterioration of rice bran. Hence, we attempted to provide the first comprehensive profiling of active serine hydrolases (SHs) present in rice bran proteome by activity-based protein profiling (ABPP) strategy. The active site-directed fluorophosphonate probe (rhodamine and biotin-conjugated) was used for the detection and identification of active SHs. ABPP revealed 55 uncharacterized active-SHs and are representing five different known enzyme families. Based on motif and domain analyses, one of the uncharacterized and miss annotated SHs (Os12Ssp, storage protein) was selected for biochemical characterization by overexpressing in yeast. The purified recombinant protein authenticated the serine protease activity in time and protein-dependent studies. Os12Ssp exhibited the maximum activity at a pH between 7.0 and 8.0. The protease activity was inhibited by the covalent serine protease inhibitor, which suggests that the ABPP approach is indeed reliable than the sequence-based annotations. Collectively, the comprehensive knowledge generated from this study would be useful in expanding the current understanding of rice bran SHs and paves the way for better utilization/stabilization of rice bran. |
| Databáze: | OpenAIRE |
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