High genetic diversity among community-associated Staphylococcus aureus in Europe: results from a multicenter study

Autor: Joana, Rolo, Maria, Miragaia, Agata Turlej Rogacka, Joanna, Empel, Ons, Bouchami, Faria, Nuno A., Ana, Tavares, Waleria, Hryniewicz, Fluit, Ad C., De Lencastre, H., Nashev, D., Melter, O., Zemlicková, H., Fridrichova, M., Westh, H., Salmenlinna, S., Lina, G., Spiliopoulou, I., Drougka, E., Kristóf, K., Rozgonyi, F., Raponi, Giammarco, Ghezzi, Maria Cristina, Wulf, M., Codita, I., Ionescu, G., Nica, M., Lísková, A., Ruiz Garbajosa, P., Canton, R., Dominguez, M. a., Petersson, A. c., Walker, R., Anderson, R., Andrews, J.
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Male
Epidemiology
medicine.disease_cause
Polymerase Chain Reaction
Staphylococci
0303 health sciences
education.field_of_study
Molecular Epidemiology
Multidisciplinary
respiratory system
Staphylococcal Infections
Bacterial Pathogens
Bacterial Typing Techniques
Electrophoresis
Gel
Pulsed-Field
Community-Acquired Infections
Europe
Infectious Diseases
Medical Microbiology
Medicine
Female
Research Article
Skin Infections
Methicillin-Resistant Staphylococcus aureus
Staphylococcus aureus
Science
Population
Microbial Sensitivity Tests
Biology
Staphylococcal infections
Microbiology
Infectious Disease Epidemiology
03 medical and health sciences
Arginine catabolic mobile element
medicine
Humans
education
Microbial Pathogens
030304 developmental biology
Genetic diversity
Population Biology
030306 microbiology
SCCmec
Genetic Variation
biochemical phenomena
metabolism
and nutrition
medicine.disease
bacterial infections and mycoses
Methicillin-resistant Staphylococcus aureus
Genetics
Population
Multilocus sequence typing
Panton–Valentine leukocidin
Zdroj: PLoS ONE, Vol 7, Iss 4, p e34768 (2012)
PLoS ONE; Vol 7
PLoS ONE
ISSN: 1932-6203
Popis: BackgroundSeveral studies have addressed the epidemiology of community-associated Staphylococcus aureus (CA-SA) in Europe; nonetheless, a comprehensive perspective remains unclear. In this study, we aimed to describe the population structure of CA-SA and to shed light on the origin of methicillin-resistant S. aureus (MRSA) in this continent.Methods and findingsA total of 568 colonization and infection isolates, comprising both MRSA and methicillin-susceptible S. aureus (MSSA), were recovered in 16 European countries, from community and community-onset infections. The genetic background of isolates was characterized by molecular typing techniques (spa typing, pulsed-field gel electrophoresis and multilocus sequence typing) and the presence of PVL and ACME was tested by PCR. MRSA were further characterized by SCCmec typing. We found that 59% of all isolates were associated with community-associated clones. Most MRSA were related with USA300 (ST8-IVa and variants) (40%), followed by the European clone (ST80-IVc and derivatives) (28%) and the Taiwan clone (ST59-IVa and related clonal types) (15%). A total of 83% of MRSA carried Panton-Valentine leukocidin (PVL) and 14% carried the arginine catabolic mobile element (ACME). Surprisingly, we found a high genetic diversity among MRSA clonal types (ST-SCCmec), Simpson's index of diversity = 0.852 (0.788-0.916). Specifically, about half of the isolates carried novel associations between genetic background and SCCmec. Analysis by BURP showed that some CA-MSSA and CA-MRSA isolates were highly related, suggesting a probable local acquisition/loss of SCCmec.ConclusionsOur results imply that CA-MRSA origin, epidemiology and population structure in Europe is very dissimilar from that of USA.
Databáze: OpenAIRE
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