Prenatal vitamin C deficiency results in differential levels of oxidative stress during late gestation in foetal guinea pig brains

Autor: Maya Devi Paidi, Pernille Tveden-Nyborg, Jens Lykkesfeldt, Janne G. Schjoldager
Rok vydání: 2014
Předmět:
Antioxidant
medicine.medical_treatment
Clinical Biochemistry
Apoptosis
medicine.disease_cause
Biochemistry
1VitC
vitamin C
Antioxidants
chemistry.chemical_compound
PCR
polymerase chain reaction
Pregnancy
Vitamin C
lcsh:QH301-705.5
NT
nitrotyrosine
lcsh:R5-920
biology
GPx
glutathione peroxidase
Nitrotyrosine
Gene Expression Regulation
Developmental
Brain
GD
gestational day
DEF
deficient
Malondialdehyde
CTRL
control
Deficiency
Female
lcsh:Medicine (General)
Signal Transduction
medicine.medical_specialty
Guinea Pigs
s18
ribosomal protein 18S
Development
Article
Superoxide dismutase
Internal medicine
SOD
superoxide dismutase
medicine
Animals
Ascorbic Acid Deficiency
MDA
malondialdehyde
HNE
hydroxynonenal
Vitamin E
Organic Chemistry
Guinea pig
Endocrinology
chemistry
lcsh:Biology (General)
Prenatal Injuries
Oxidative stress
biology.protein
PFA
paraformaldehyde
Zdroj: Redox Biology, Vol 2, Iss C, Pp 361-367 (2014)
Redox Biology
ISSN: 2213-2317
DOI: 10.1016/j.redox.2014.01.009
Popis: Antioxidant defences are comparatively low during foetal development making the brain particularly susceptible to oxidative stress during antioxidant deficiencies. The brain is one of the organs containing the highest concentration of vitamin C (VitC) and VitC deficiency during foetal development may place the brain at risk of redox status imbalance. In the present study, we investigated the developmental pattern and effect of VitC deficiency on antioxidants, vitamin E and superoxide dismutase (SOD), assessed oxidative damage by measuring malondialdehyde (MDA), hydroxynonenal (HNE) and nitrotyrosine (NT) and analysed gene and protein expression of apoptosis marker caspase-3 in the guinea pig foetal brain at two gestational (GD) time points, GD 45/pre-term and GD 56/near term following either a VitC sufficient (CTRL) or deficient (DEF) maternal dietary regime. We show that except for SOD, antioxidants and oxidative damage markers are differentially expressed between the two GDs, with high VitC (p
Graphical abstract
Highlights • Maternal vitamin C deficiency affects redox balance in the foetal brain. • Differential regulation during late gestation time-points. • The foetal brain is susceptible to oxidative stress imposed by vitamin C deficiency.
Databáze: OpenAIRE
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