| Autor: |
Jian-Qiu, Jin, Qian, Wang, Yu-Xing, Zhang, Xing, Wang, Zhi-Yue, Lu, Bo-Wen, Li |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Lasers in Medical Science. 37:3461-3472 |
| ISSN: |
1435-604X |
| DOI: |
10.1007/s10103-022-03607-y |
| Popis: |
Early treatment of oral precancerous lesions is considered as a key strategy for in oral carcinogenesis prevention. Increasing evidence has suggested that the transforming growth factor beta (TGF-β) signaling pathway is tightly involved in the process of oral-carcinogenesis. In this study, we investigated the inhibition effect and potential mechanism of 5-aminolaevulinic acid photodynamic therapy (ALA-PDT) in human oral precancerous cells via TGF-β pathway.Here, the dysplastic oral keratinocyte (DOK) cells were incubated with ALA concentration of 1 mM/mL for 4 h and then irradiated with a Helium-Neon (He-Ne) ion laser at 633 nm (200 mW/cmThe TGF-β signaling could exert in suppressive effects on DOK cells after ALA-PDT. The cell proliferation and migration rate of DOK cells was significantly reduced and apoptosis and ROS generation induced more effectively by ALA-PDT combined with LY2109761. Furthermore, cell cycle analysis revealed that the combined treatment resulted in G0/G1 phase arrest.ALA-PDT suppresses the growth of oral precancerous cells by regulating the TGF-β signaling pathway, and its suppressive effect was enhanced using LY2109761. These results indicate that it could be a promising alternative treatment against oral precancerous lesions. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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