Nicotine Induces Polyspermy in Sea Urchin Eggs through a Non-Cholinergic Pathway Modulating Actin Dynamics
| Autor: | Filip Vasilev, Jong Tai Chun, Luigia Santella, Nunzia Limatola |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Carbachol non-cholinergic cofilin Receptors Nicotinic Article sea urchin Nicotine 03 medical and health sciences 0302 clinical medicine medicine Animals Humans lcsh:QH301-705.5 Acetylcholine receptor calcium Chemistry General Medicine Polyspermy Spermatozoa Actins 3. Good health Cell biology 030104 developmental biology Nicotinic agonist lcsh:Biology (General) fertilization Sea Urchins 030220 oncology & carcinogenesis Oocytes Cholinergic polyspermy actin Biomarkers Acetylcholine Signal Transduction medicine.drug Ionotropic effect nicotine |
| Zdroj: | Cells, Vol 9, Iss 1, p 63 (2019) Cells Volume 9 Issue 1 |
| ISSN: | 2073-4409 |
| Popis: | While alkaloids often exert unique pharmacological effects on animal cells, exposure of sea urchin eggs to nicotine causes polyspermy at fertilization in a dose-dependent manner. Here, we studied molecular mechanisms underlying the phenomenon. Although nicotine is an agonist of ionotropic acetylcholine receptors, we found that nicotine-induced polyspermy was neither mimicked by acetylcholine and carbachol nor inhibited by specific antagonists of nicotinic acetylcholine receptors. Unlike acetylcholine and carbachol, nicotine uniquely induced drastic rearrangement of egg cortical microfilaments in a dose-dependent way. Such cytoskeletal changes appeared to render the eggs more receptive to sperm, as judged by the significant alleviation of polyspermy by latrunculin-A and mycalolide-B. In addition, our fluorimetric assay provided the first evidence that nicotine directly accelerates polymerization kinetics of G-actin and attenuates depolymerization of preassembled F-actin. Furthermore, nicotine inhibited cofilin-induced disassembly of F-actin. Unexpectedly, our results suggest that effects of nicotine can also be mediated in some non-cholinergic pathways. |
| Databáze: | OpenAIRE |
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