Characterization of the chromosomal aac(6')-Ic gene from Serratia marcescens
| Autor: | P N Rather, H. Munayyer, K J Shaw, Paul A. Mann, P. Bennett, G. L. Petrikkos, Roberta S. Hare, R Mierzwa, F J Sabatelli, P. Downey, George H. Miller |
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| Rok vydání: | 1992 |
| Předmět: |
DNA
Bacterial Serratia Sequence analysis Molecular Sequence Data Restriction fragment Plasmid Coding region Pharmacology (medical) Gene Chromatography High Pressure Liquid Palindromic sequence Pharmacology Genetics biology Base Sequence Nucleic acid sequence biochemical phenomena metabolism and nutrition Chromosomes Bacterial Molecular biology Infectious Diseases Subcloning biology.protein bacteria Hybridization Genetic DNA Probes Polymorphism Restriction Fragment Length Plasmids Research Article |
| Zdroj: | Antimicrobial agents and chemotherapy. 36(7) |
| ISSN: | 0066-4804 |
| Popis: | The DNA sequence of the chromosomal aac(6')-Ic gene from Serratia marcescens, which had been previously cloned (H. M. Champion, P. M. Bennett, D. A. Lewis, and D. S. Reeves, J. Antimicrob. Chemother. 22:587-596, 1988) was determined. High-pressure liquid chromatographic analysis of extracts prepared from Escherichia coli carrying the chromosomal aac(6')-Ic gene on a plasmid confirmed the presence of 6'-N-acetyltransferase activity in this strain, which was suggested by the aminoglycoside resistance profile. DNA sequence analysis of the cloned 2,057-bp PstI fragment revealed several regions of homology to previously characterized sequences from GenBank, including the rpoD and tRNA-2 genes of E. coli. Subcloning experiments confirmed the coding sequence of the aac(6')-Ic gene to be at positions 1554 to 1992. The predicted amino acid sequence of the AAC(6')-Ic protein suggested that it was the third member of a family of AAC(6') proteins which included a coding region identified between the aadB and aadA genes of Tn4000 and an AAC(6') protein encoded by pUO490, which was isolated from Enterobacter cloacae. Primer extension analysis suggested that the -35 region of the aac(6')-Ic promoter overlapped a large palindromic sequence which may be involved in the regulation of the aac(6')-Ic gene. Hybridization experiments utilizing a restriction fragment from the aac(6')-Ic gene showed that all S. marcescens organisms carried this gene whether or not the AAC(6')-I resistance profile was expressed. Organisms other than Serratia spp. did not hybridize to this probe. |
| Databáze: | OpenAIRE |
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