Alterations of Extracellular Matrix Mechanical Properties Contribute to Age-Related Functional Impairment of Human Skeletal Muscles

Autor: Piero G. Pavan, Lorenzo Marcucci, Carlo Reggiani, Elena Monti, Marco Narici, Carla Stecco, Michela Bondì, Chenglei Fan
Rok vydání: 2020
Předmět:
Male
collagen
0301 basic medicine
Functional impairment
Muscle Fibers
Skeletal
Sarcomere
Picrosirius red
lcsh:Chemistry
Extracellular matrix
0302 clinical medicine
single muscle fiber
lcsh:QH301-705.5
Spectroscopy
Aged
80 and over
Chemistry
General Medicine
Middle Aged
Biomechanical Phenomena
Computer Science Applications
medicine.anatomical_structure
Female
Elongation
Adult
Sarcomeres
medicine.medical_specialty
Adolescent
extracellular matrix
Article
Catalysis
Inorganic Chemistry
Young Adult
03 medical and health sciences
aging
resting tension
skeletal muscle
Internal medicine
Age related
medicine
Humans
Physical and Theoretical Chemistry
Muscle
Skeletal
Molecular Biology
Aged
Organic Chemistry
Skeletal muscle
Compliance (physiology)
030104 developmental biology
Endocrinology
lcsh:Biology (General)
lcsh:QD1-999
Stress
Mechanical
030217 neurology & neurosurgery
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 11
International Journal of Molecular Sciences, Vol 21, Iss 3992, p 3992 (2020)
ISSN: 1422-0067
Popis: Aging of human skeletal muscles is associated with increased passive stiffness, but it is still debated whether muscle fibers or extracellular matrix (ECM) are the determinants of such change. To answer this question, we compared the passive stress generated by elongation of fibers alone and arranged in small bundles in young healthy (Y: 21 years) and elderly (E: 67 years) subjects. The physiological range of sarcomere length (SL) 2.5&ndash
3.3 &mu
m was explored. The area of ECM between muscle fibers was determined on transversal sections with picrosirius red, a staining specific for collagen fibers. The passive tension of fiber bundles was significantly higher in E compared to Y at all SL. However, the resistance to elongation of fibers alone was not different between the two groups, while the ECM contribution was significantly increased in E compared to Y. The proportion of muscle area occupied by ECM increased from 3.3% in Y to 8.2% in E. When the contribution of ECM to bundle tension was normalized to the fraction of area occupied by ECM, the difference disappeared. We conclude that, in human skeletal muscles, the age-related reduced compliance is due to an increased stiffness of ECM, mainly caused by collagen accumulation.
Databáze: OpenAIRE
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