Adaptive mutation: General mutagenesis is not a programmed response to stress but results from rare coamplification of dinB with lac
| Autor: | John R. Roth, Eric Kofoid, Dan I. Andersson, Kim L. Bunny, Elisabeth Kugelberg, E. Susan Slechta |
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| Rok vydání: | 2003 |
| Předmět: |
Salmonella typhimurium
Time Factors Reversion lac operon Lactose Biology Bacterial Proteins Adaptive mutation Gene duplication Selection Genetic Gene DNA Polymerase beta Genetics Multidisciplinary Escherichia coli Proteins Mutagenesis Biological Sciences Molecular biology Lac Operon Conjugation Genetic Mutation (genetic algorithm) DNA polymerase IV Gene Deletion Genome Bacterial Plasmids |
| Zdroj: | Proceedings of the National Academy of Sciences. 100:12847-12852 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1735464100 |
| Popis: | In a particular genetic system, selection stimulates reversion of a lac mutation and causes genome-wide mutagenesis (adaptive mutation). Selection allows rare plated cells with a duplication of the leaky lac allele to initiate clones within which further lac amplification improves growth rate. Growth and amplification add mutational targets to each clone and thereby increase the likelihood of reversion. We suggest that general mutagenesis occurs only in clones whose lac amplification includes the nearby dinB + gene (for error-prone DNA polymerase IV). Thus mutagenesis is not a programmed response to stress but a side effect of amplification in a few clones; it is not central to the effect of selection on reversion. |
| Databáze: | OpenAIRE |
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